ZFIN ID: ZDB-FIG-200323-39
Gordon et al., 2019 - Fenestrae-associated protein Plvap regulates the rate of blood-borne proteins passage into the hypophysis. Development (Cambridge, England)   146(23) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Adult

Fig. 4 Developmental expression of Plvap orthologs in the hypophyseal vasculature. (A) Schematic representation of the predicted secondary structure of zebrafish (Plvapa and Plvapb) and mammalian (PLVAP) proteins. (B) A scheme describing the major vasculature of the larval hypophysis and brain. (C,D) Whole-mount fluorescence in situ hybridization of zebrafish larvae at 3 or 5 dpf showing restricted plvapa (C) and plvapb (D) mRNA expression in the hypophyseal but not in the brain vasculature. Scale bars: 5 µm. (E,F) Whole-mount FISH of adult zebrafish showing restricted plvapa (E) and plvapb (F) mRNA expression in the hypophyseal but not in the brain vasculature. Scale bars: 20 µm. AMCtA, anterior (rostral) mesencephalic central artery; BCA, basal communicating artery; CrDI, cranial division of the internal carotid artery; HypA, hypophyseal artery; HypV, hypophyseal vein; PLA, palatocerebral artery. See related Figs S2 and S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
plvapa WT standard conditions Protruding-mouth Not Detected brain vasculature blood vessel endothelium ISH
Protruding-mouth Not Detected hypophyseal capillary blood vessel endothelium ISH
Day 5 Not Detected brain vasculature blood vessel endothelium ISH
Day 5 hypophyseal capillary blood vessel endothelium ISH
Adult hypophyseal capillary blood vessel endothelium ISH
Adult Not Detected telencephalon blood vessel endothelium ISH
plvapb WT standard conditions Protruding-mouth Not Detected brain vasculature blood vessel endothelium ISH
Protruding-mouth hypophyseal capillary blood vessel endothelium ISH
Day 5 Not Detected brain vasculature blood vessel endothelium ISH
Day 5 hypophyseal capillary blood vessel endothelium ISH
Adult hypophyseal capillary blood vessel endothelium ISH
Adult Not Detected telencephalon blood vessel endothelium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development