ZFIN ID: ZDB-FIG-200130-9
Huning et al., 2020 - Two paralogous znf143 genes in zebrafish encode transcriptional activator proteins with similar functions but expressed at different levels during early development. BMC molecular and cell biology   21:3 Full text @ BMC Mol Cell Biol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 3

Similar spatial expression of znf143a and znf143b in 24hpf embryos. Antisense digoxigenin riboprobes against the znf143a and znf143b were used for whole-mount in situ hybridization assays. The probe for znf143b targeted the last exon of the coding region (approximately 186 nt corresponding to the last 61 amino acids) and the 3’UTR of the gene, while the probe for znf143a was designed solely against the 3’UTR. Embryos used were fixed and stained at 24hpf. The panels illustrate views from four different embryos for each probe, with the right-most panels showing an enlarged dorsal view of the head. We note that in all experiments, the znf143b probe produced fainter staining, most likely because of a smaller than optimal riboprobe that was necessary to ensure gene specificity. Specific head, brain and neural structures are identified with the following abbreviations: f, forebrain; m, midbrain; h, hindbrain; mhb, midbrain hindbrain boundary; c, cerebellum; scn, spinal cord neurons

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
znf143a AB/TL standard conditions Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
znf143b AB/TL standard conditions Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC molecular and cell biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Mol Cell Biol