ZIKV favors monocyte arrest in zebrafish vessels, but does not affect their arresting time. Human primary monocytes from three donors were treated and injected as in Fig. 7a, and imaged with an epifluorescence microscope immediately after injection (0–5 min). a Micrographs representing the type of movement observed (Supplementary Movie S2). White arrowheads indicate examples of monocytes circulating (left panels), stopping (middle panels), or undergoing a rolling-like movement (right panels). Note that timescales are different for each behavior. The yellow arrow represents the direction of the bloodstream. Scale bar: 40 µm. b–g Monocytes were automatically tracked over time. b The track duration (in seconds) of each monocyte was quantified. Each dot corresponds to a single monocyte tracked over ≈5 min and the red line represents the mean (NI n = 2 fish; ZIKV n = 3 fish; ADE–ZIKV n = 3 fish). c Color-coded representation of monocyte speed in the zebrafish vasculature. Red tracks indicate arrested monocytes. d, e Quantification of the mean track speed of monocytes. Each red dot corresponds to a single monocyte tracked over ≈5 min. d The box plots are representative of the data of one fish. The center line is the median, the box extends from 25th to 75th percentiles, and the whiskers include from the smallest to the largest value. e The bar graph shows the mean ± SD of NI n = 2 fish, ZIKV n = 3 fish, and ADE–ZIKV n = 3 fish per condition. Each dot is one tracked monocyte. Quantification of f the percentage and g the arrest length (in seconds) among the monocytes that arrested for more than 2 s. f, g The bar graphs represent the mean ± SEM. Each dot represents the mean of all monocytes tracked in a given fish (NI n = 2 fish (139 tracks), ZIKV n = 3 fish (269 tracks), and ADE–ZIKV n = 3 fish (316 tracks)). Two-tailed p value was ns = nonsignificant, <0.05 (*), <0.005 (**), and <0.0001 (***). Statistical significance was determined by using a t test. Source data in b, e, g are provided as a Source Data file
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