Delayed stress granule formation in zebrafish larvae. (A) Representative images showing the formation of stress granules in the midbrain (optical tectum) at indicated time with heat shock at 42°C for GFP–G3BP1 knock-in fish at different ages during early development. Scale bars: 10 µm. (B) Quantification of the number of stress granules (sized ≥1 µm) at the same depth (20 µm under epidermis) in fish from 1 to 11 dpf as indicated. Values represent mean±s.e.m.; n=4 zebrafish for each age, with 100–300 cells scored for each fish. Statistical results analyzed by two-way ANOVA followed by multiple comparison are shown in the table to the right. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.
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