ZFIN ID: ZDB-FIG-191230-1886
Klingseisen et al., 2019 - Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS. Developmental Cell   51(6):730-744.e6 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Pec-fin

Figure 5

Neurofascin B Regulates Myelin Targeting during the Critical Period of Sheath Formation and Retraction

(A–A″′) Single time-point images of an individual oligodendrocyte in a wild-type animal as its myelinating processes exhibit several distinct dynamic activities over time: 1=sheath formation, 2=slow sheath growth, 3=rapid sheath growth, 4=exploratory process retraction, 5=engagement with a cell body, 6=retraction from a cell body, 7=retraction from an axon. Scale bar, 10 μm.

(B and B′) Single time-point confocal images of an individual oligodendrocyte in a wildtype animal as it is forming myelin sheaths at the beginning (A) and toward the end (A′) of the critical period. Scale bar, 10 μm.

(C and C′) Single time-point confocal images of an individual oligodendrocyte in a nfascb MO-injected animal as it is forming myelin sheaths at the beginning (A) and toward the end (A′) of the critical period. Note that a cell body has become myelinated during this time (arrows). Scale bar, 10 μm.

(D–D″′) Time series showing myelination of a cell body in a nfascb MO-injected animal. Scale bar, 10 μm.

(E–E″′) Time series showing a myelinating process being retracted from a cell body in a control. Scale bar, 10 μm.

(F) Number of myelin sheaths generated on axons per oligodendrocyte during time-lapse analyses of control and nfascb MO-injected animals (control mean 13.50 ± 2.91 SD, n = 20 oligodendrocytes from 13 animals, nfascb MO mean 12.35 ± 3.30 SD, n = 34 oligodendrocytes from 17 animals, p = 0.2041, t test).

(G) Graph showing the duration between the first formed and the last formed myelin sheath by individual oligodendrocytes in control and nfascb MO-injected animals (control mean 3.55 ± 1.45 SD, n = 13 oligodendrocytes from 10 animals, nfascb MO mean 4.11 ± 2.30 SD, n = 29 oligodendrocytes from 21 animals, p = 0.4249, t test).

(H) Time of cell body myelination during time-lapse of control (none were myelinated) and nfascb MO-injected animals. Cell bodies are myelinated by Nfasc B-depleted cells during the same period as axons.

(I) Number of myelin sheaths retracted from axons per oligodendrocyte during time-lapse of control and nfascb MO-injected animals (control mean 2.81 ± 1.60 SD, n = 16 oligodendrocytes from 13 animals, nfascb MO mean 2.81 ± 1.27 SD, n = 26 oligodendrocytes from 17 animals, p = 0.7463, Mann–Whitney test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ue102Tg; ue7Tg standard conditions Pec-fin oligodendrocyte myelin sheath IFL
Pec-fin spinal cord oligodendrocyte IFL
ue102Tg; ue7Tg + MO1-nfascb standard conditions Pec-fin oligodendrocyte myelin sheath IFL
Pec-fin spinal cord oligodendrocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ue102Tg; ue7Tg + MO1-nfascb standard conditions Pec-fin spinal cord central nervous system myelin formation process quality, abnormal
Pec-fin spinal cord myelin sheath surrounding spinal cord neuronal cell body, abnormal
Pec-fin spinal cord oligodendrocyte has normal numbers of parts of type oligodendrocyte myelin sheath, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 51(6), Klingseisen, A., Ristoiu, A.M., Kegel, L., Sherman, D.L., Rubio-Brotons, M., Almeida, R.G., Koudelka, S., Benito-Kwiecinski, S.K., Poole, R.J., Brophy, P.J., Lyons, D.A., Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS, 730-744.e6, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell