|ZFIN ID: ZDB-FIG-191230-1886|
Neurofascin B Regulates Myelin Targeting during the Critical Period of Sheath Formation and Retraction
(A–A″′) Single time-point images of an individual oligodendrocyte in a wild-type animal as its myelinating processes exhibit several distinct dynamic activities over time: 1=sheath formation, 2=slow sheath growth, 3=rapid sheath growth, 4=exploratory process retraction, 5=engagement with a cell body, 6=retraction from a cell body, 7=retraction from an axon. Scale bar, 10 μm.
(B and B′) Single time-point confocal images of an individual oligodendrocyte in a wildtype animal as it is forming myelin sheaths at the beginning (A) and toward the end (A′) of the critical period. Scale bar, 10 μm.
(C and C′) Single time-point confocal images of an individual oligodendrocyte in a
(D–D″′) Time series showing myelination of a cell body in a
(E–E″′) Time series showing a myelinating process being retracted from a cell body in a control. Scale bar, 10 μm.
(F) Number of myelin sheaths generated on axons per oligodendrocyte during time-lapse analyses of control and
(G) Graph showing the duration between the first formed and the last formed myelin sheath by individual oligodendrocytes in control and
(H) Time of cell body myelination during time-lapse of control (none were myelinated) and
(I) Number of myelin sheaths retracted from axons per oligodendrocyte during time-lapse of control and
|GFP||ue102Tg; ue7Tg||standard conditions||Pec-fin||IFL|
|ue102Tg; ue7Tg + MO1-nfascb||standard conditions||Pec-fin||IFL|
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article.
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Reprinted from Developmental Cell, 51(6), Klingseisen, A., Ristoiu, A.M., Kegel, L., Sherman, D.L., Rubio-Brotons, M., Almeida, R.G., Koudelka, S., Benito-Kwiecinski, S.K., Poole, R.J., Brophy, P.J., Lyons, D.A., Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS, 730-744.e6, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell