ZFIN ID: ZDB-FIG-191230-1882
Klingseisen et al., 2019 - Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS. Developmental Cell   51(6):730-744.e6 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Figure 1

Neurofascin B Is Required for Myelin Targeting in the Zebrafish CNS

(A and B) Images of wild type (A) and ue56 mutant (B) larvae at 5 days post-fertilization (dpf), showing normal morphological development of ue56 mutants. Scale bar, 500 μm.

(C–D′) Confocal images of myelin in a wildtype larva (C and C′) and ue56 mutant (D and D′) at 5dpf, visualized using Tg(mbp:EGFP-CAAX). Lateral views, with anterior to the left. (C) and (D) are maximum intensity projections of z sections taken through the entire spinal cord, with (C′) and (D′) being a projection of a subset of z sections centered closer to the midline in the region where cell bodies are prominent and myelinated in mutants. Scale bars, 20 μm.

(E) Quantitation of myelinated cell body number in wildtype and ue56 mutants at 5dpf in a 3 somite long stretch of spinal cord (wild type median = 2, 25th percentile 0, 75th percentile 2.5, n = 13 animals, ue56 median = 53, 25th percentile 46.5, 75th percentile 62.75, n = 14 animals, p < 0.0001, t test)

(F) Sequence at amino acid positions where the ue56 mutation generates a stop codon and indication of the conserved region across species.

(G) Schematic of predicted domain structure of mouse neurofascin 186, neurofascin 155, and zebrafish neurofascin B, with indication of where the mutation in ue56 mutants resides.

(H–I′) Z projections of confocal images of neurons (red) and myelin (green) in a wildtype animal (H and H′) and nfascbue56 mutant (I and I′), showing myelin sheaths made on axons (H and I) and myelination of cell bodies in mutants in a region with cell bodies only (H′ versus I′). Scale bars, 20 μm.

(J) Schematic cross section of the larval zebrafish spinal cord denoting areas with cell bodies (red) and myelinated axons (green) (Top panel). Dorso-ventral and medio-lateral positions of all myelinated cell bodies (dots) in a 3-somite long stretch of spinal cord of 11 mutants (bottom panel).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nfascbue56/ue56; ue2Tg standard conditions Day 5 oligodendrocyte myelin sheath IFL
Day 5 spinal cord myelin sheath IFL
nfascbue56/ue56; ue2Tg; ue3Tg standard conditions Day 5 oligodendrocyte myelin sheath IFL
Day 5 spinal cord myelin sheath IFL
ue2Tg standard conditions Day 5 oligodendrocyte myelin sheath IFL
Day 5 spinal cord myelin sheath IFL
ue2Tg; ue3Tg standard conditions Day 5 oligodendrocyte myelin sheath IFL
Day 5 spinal cord myelin sheath IFL
mCherry nfascbue56/ue56; ue2Tg; ue3Tg standard conditions Day 5 glial cell (sensu Vertebrata) IFL
Day 5 spinal cord neuron IFL
ue2Tg; ue3Tg standard conditions Day 5 spinal cord neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nfascbue56/ue56; ue2Tg standard conditions Day 5 spinal cord central nervous system myelin formation process quality, abnormal
Day 5 spinal cord myelin sheath surrounding spinal cord axon, normal
Day 5 spinal cord myelin sheath surrounding spinal cord neuronal cell body, abnormal
nfascbue56/ue56; ue2Tg; ue3Tg standard conditions Day 5 spinal cord central nervous system myelin formation process quality, abnormal
Day 5 spinal cord myelin sheath surrounding spinal cord axon, normal
Day 5 spinal cord myelin sheath surrounding spinal cord neuronal cell body, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 51(6), Klingseisen, A., Ristoiu, A.M., Kegel, L., Sherman, D.L., Rubio-Brotons, M., Almeida, R.G., Koudelka, S., Benito-Kwiecinski, S.K., Poole, R.J., Brophy, P.J., Lyons, D.A., Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS, 730-744.e6, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell