ZFIN ID: ZDB-FIG-191230-1482
Duchemin et al., 2019 - Mechanically activated Piezo channels modulate outflow tract valve development through the Yap1 and Klf2-Notch signaling axis. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Figure 7 Klf2a and notch are necessary for valve formation. Quantification of the valve phenotypes at 72 hpf, 96 hpf and 120 hpf (normal, thick, delayed) in klf2a+/+(n = 11), and klf2a-/- (n = 12) using the Tg(fli:gal4FF/UAS:Kaede). (A) and notch1b+/+ (n = 7) and notch1b-/- (n = 20) using Tg(fli:lifeact-EGFP; kdrl:nls-mCherry) embryos. (B) Scale bar: 10 µm. N = 3 independent experiments. (C) Confocal z-sections of the Tg(tp1:dGFP) in klf2a+/+ and klf2a-/- embryos at 72 hpf, 96 hpf and 120 hpf. V: ventricle. Scale bar: 10 µm. (C’) Quantification of the fluorescent intensity of the Notch reporter (GFP over background) in the anterior versus posterior parts of the valves in in klf2a+/+ (n = 5) and klf2a-/- (n = 4) embryos. Statistical test were performed to compare the posterior intensities in klf2a+/+versus klf2a-/- at 72 hpf (p=0,05), 96 hpf (p=0,03) and 120 hpf (p=0,04). Student’s t-test. Boxplot: Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. Results obtained from three independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP klf2aig4/ig4; um14Tg standard conditions Protruding-mouth bulbus arteriosus endocardial cushion IFL
Day 4 bulbus arteriosus endocardial cushion IFL
Day 5 ventriculo bulbo valve anterior region IFL
Day 5 ventriculo bulbo valve posterior region IFL
notch1bsa11236/sa11236; y173Tg; zf495Tg standard conditions Protruding-mouth bulbus arteriosus endocardial cushion IFL
Day 4 bulbus arteriosus endocardial cushion IFL
Day 5 ventriculo bulbo valve IFL
um14Tg standard conditions Protruding-mouth bulbus arteriosus endocardial cushion IFL
Day 4 bulbus arteriosus endocardial cushion IFL
Day 5 ventriculo bulbo valve anterior region IFL
Day 5 ventriculo bulbo valve posterior region IFL
y173Tg; zf495Tg standard conditions Protruding-mouth bulbus arteriosus endocardial cushion IFL
Day 4 bulbus arteriosus endocardial cushion IFL
Day 5 ventriculo bulbo valve IFL
Kaede rk8Tg; ubs3Tg standard conditions Protruding-mouth bulbus arteriosus vascular endothelium IFL
Day 4 bulbus arteriosus vascular endothelium IFL
Day 5 bulbus arteriosus vascular endothelium IFL
ubs3Tg ; rk8Tg ; klf2aig4/ig4 standard conditions Protruding-mouth bulbus arteriosus vascular endothelium IFL
Day 4 bulbus arteriosus vascular endothelium IFL
Day 5 bulbus arteriosus vascular endothelium IFL
mCherry notch1bsa11236/sa11236; y173Tg; zf495Tg standard conditions Protruding-mouth bulbus arteriosus endocardial cushion IFL
Day 4 bulbus arteriosus endocardial cushion IFL
Day 5 ventriculo bulbo valve nucleus IFL
y173Tg; zf495Tg standard conditions Protruding-mouth bulbus arteriosus endocardial cushion IFL
Day 4 bulbus arteriosus endocardial cushion IFL
Day 5 ventriculo bulbo valve nucleus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
klf2aig4/ig4; um14Tg standard conditions Protruding-mouth bulbus arteriosus endocardial cushion EGFP expression decreased amount, abnormal
Protruding-mouth ventriculo bulbo valve Notch signaling involved in heart development decreased process quality, abnormal
Day 4 bulbus arteriosus endocardial cushion EGFP expression decreased amount, abnormal
Day 4 ventriculo bulbo valve Notch signaling involved in heart development decreased process quality, abnormal
Day 5 ventriculo bulbo valve Notch signaling involved in heart development decreased process quality, abnormal
Day 5 ventriculo bulbo valve posterior region EGFP expression decreased amount, abnormal
notch1bsa11236/sa11236; y173Tg; zf495Tg standard conditions Protruding-mouth ventriculo bulbo valve morphogenesis delayed, abnormal
Day 4 ventriculo bulbo valve malformed, abnormal
Day 4 ventriculo bulbo valve morphogenesis decreased process quality, abnormal
Day 5 ventriculo bulbo valve malformed, abnormal
Day 5 ventriculo bulbo valve morphogenesis decreased process quality, abnormal
ubs3Tg ; rk8Tg ; klf2aig4/ig4 standard conditions Protruding-mouth ventriculo bulbo valve morphogenesis delayed, abnormal
Day 4 ventriculo bulbo valve morphogenesis delayed, abnormal
Day 5 ventriculo bulbo valve morphogenesis delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife