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Fig. 12

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ZDB-FIG-191220-40

Publication

Vetrivel et al., 2019 - Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia

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Fig. 12

Hist2h3ca1 knock-down affects zebrafish eye development. a–c: After MO-mediated knockdown of zebrafish hist2h3ca1, FGF signaling (green EGFP reporter) was still preserved in telencephalic (te), otic vesicle (ov) and midbrain-hindbrain-boundary (mhb) regions, but lost in the lens (dashed circle) of morphant embryos (c), compared to not injected (a) and mismMO-injected controls (b), analyzed at 30 hpf (hours post-fertilization). re: retina. d–f: At 30 hpf, expression of cryba2b (red) was almost completely lost in the lens (le) of morphants (hMO) (f), compared to not injected (n.i.) (d) and mismatched (mMO) (e) controls, while isl1 expression (green) was still present in the retina (re). g–i: TGFb (TGFβ) signaling (green EGFP reporter) was activated in the brain, retina (re) and lens (le) of not injected and control-injected embryos (g, h), while it was specifically absent in the lens region (dashed circle) of morphant embryos (i) at 2 dpf (day post-fertilization). All panels display lateral views of zebrafish cephalic regions, with anterior to the left. Displayed phenotypes are representative of n = 60 embryos per condition. The scale bars are 100 μm in A and G, 50 μm in D and apply to all images in the same row.

Expression Data

Genes:	cryba2b d2EGFP EGFP isl1a
Fish:	WT WT + MO1-hist2h3ca1 ia16Tg pt6Tg ia16Tg + MO1-hist2h3ca1 pt6Tg + MO1-hist2h3ca1
Knockdown Reagent:	MO1-hist2h3ca1
Anatomical Terms:	brain lens midbrain hindbrain boundary otic vesicle retina telencephalon
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-hist2h3ca1 ia16Tg + MO1-hist2h3ca1 pt6Tg + MO1-hist2h3ca1
Knockdown Reagent:	MO1-hist2h3ca1
Observed In:	lens lens development in camera-type eye
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Experimental Eye Research, 188, Vetrivel, S., Tiso, N., Kügler, A., Irmler, M., Horsch, M., Beckers, J., Hladik, D., Giesert, F., Gailus-Durner, V., Fuchs, H., Sabrautzki, S., German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Neuherberg, Germany, Adler, T., Treise, I., Busch, D.H., Aguilar-Pimentel, A., Ollert, M., Götz, A., Amarie, O.V., Stoeger, T., Schulz, H., Becker, L., Klopstock, T., Schrewe, A., Spielmann, N., Bekeredjian, R., Garrett, L., Hölter, S.M., Zimprich, A., Wurst, W., Mayer-Kuckuk, P., Hans, W., Rozman, J., Klingenspor, M., Neff, F., da Silva-Buttkus, P., Calzada-Wack, J., Rácz, I., Zimmer, A., Rathkolb, B., Wolf, E., Prehn, C., Adamski, J., Östereicher, M., Miller, G., Steinkamp, R., Lengger, C., Maier, H., Stoeger, C., Leuchtenberger, S., Gailus-Durner, V., Fuchs, H., Hrabě de Angelis, M., Hrabě de Angelis, M., Graw, J., Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia, 107632, Copyright (2019) with permission from Elsevier. Full text @ Exp. Eye. Res.