ZFIN ID: ZDB-FIG-191025-3
Li et al., 2019 - Reversion of tumor hepatocytes to normal hepatocytes during liver tumor regression in an oncogene transgenic zebrafish model. Disease models & mechanisms   12(10) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 2

Tracing liver tumor cells during tumor regression in adult fish. (A) Flowchart of fish treatment and sample collection. CreER and CreER/xmrk adult fish (4 mpf) were treated with Dox for 6 weeks to induce HCC. 4-OHT was added at 5 wpi (weeks post-induction) for 3 days to cause loxPrecombination for marking the tumor cell lineage with RFP expression. Fish were collected at 6 wpi, 1 wpr (weeks post-regression), 2 wpr and 4 wpr for various assays. (B) Fluorescent protein expression and histological analyses of livers. Rows 1 and 2 are representative images of gross observation of GFP and RFP in the livers under a stereomicroscope. Row 3 shows representative confocal images of the fresh liver tissue immersed in phosphate buffer to show the re-establishment of the two-cell hepatic plate structure from tumor-reverted hepatocytes during tumor regression. Row 4 shows representative images of fixed liver sections for GFP expression, RFP expression (stained with anti-RFP antibody) and nuclear staining by DAPI for quantification of cell number. Row 5 shows representative images of H&E staining. Black arrows, large and irregular nuclei; yellow arrows, prominent nucleoli. (C) Quantification of percentages of GFP+ hepatocytes and RFP+ hepatocytes in total hepatocytes based on liver sections from panel B row 3 (n=10). (D) Quantification of 2D liver size based on the left lateral view of dissected fish as shown in row 2 of panel B (n=10/group). *P<0.05.


Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz25Tg; gz38Tg chemical treatment by environment: afimoxifene, chemical treatment by environment: doxycycline Adult liver IHC
Adult liver hepatic tumor IHC
gz38Tg chemical treatment by environment: afimoxifene, chemical treatment by environment: doxycycline Adult Not Detected liver IHC
EGFP gz25Tg; gz38Tg chemical treatment by environment: afimoxifene, chemical treatment by environment: doxycycline Adult liver IHC
gz38Tg chemical treatment by environment: afimoxifene, chemical treatment by environment: doxycycline Adult liver IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz25Tg; gz38Tg chemical treatment by environment: afimoxifene, chemical treatment by environment: doxycycline Adult liver hepatocellular carcinoma increased amount, abnormal
Adult liver increased size, abnormal
Adult liver neoplastic, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.