Figure 7—figure supplement 1.

Validation of automated detection of neuronal cell nuclei in larval (five dpf) zebrafish.

75 × 75×75 µm regions were selected on each fish to validate detection of nuclei: sample regions R1, R2 and R3 are presented (A). 3D visualizations of thresholded original data (B–a) and nuclear probabilities based on the trained classifier (B–b) in Region two are shown. 3D rendering of validation is presented in (B–c); green denotes true positive, blue false positive, orange false negative. Good agreement between manual and automated segmentation is shown on regional 3D rendering and f1 scores (C) across all specimens.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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