ZFIN ID: ZDB-FIG-190723-684
Kiselev et al., 2019 - Truncating Variant in Myof Gene Is Associated With Limb-Girdle Type Muscular Dystrophy and Cardiomyopathy. Frontiers in genetics   10:608 Full text @ Front Genet
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Figure 3

Loss of Myof affects the skeletal muscle of zebrafish embryos. (A)myof knockdown was achieved by injecting a splice-target morpholino (myof-MO). (B) Loss of Myof results in wavy and abnormally patterned myofibers (Myosin, red), and disruption of the myosepta, seen by a decreased intensity in the staining and increased width (Dystrophin and Dystroglycan, green). (C)Analysis of the myofiber ultrastructure by electron microscopy shows disruption of myofiber structure (C4) and their sarcomeres (arrows, C5, C6), abnormally shaped and distributed mitochondria (arrowheads, C5), when compared to control mitochondria (arrowheads, C1), and Z-disc thickening (arrowheads, C6).

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dag1 Ab4-dag1 WT + MO1-myof standard conditions Long-pec myoseptum IHC
dmd Ab1-dmd WT + MO1-myof standard conditions Long-pec myoseptum IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myof standard conditions Long-pec myoseptum dag1 expression increased distribution, abnormal
Long-pec myoseptum dmd expression increased distribution, abnormal
Long-pec myoseptum increased width, abnormal
Long-pec skeletal muscle cell mitochondrion morphology, abnormal
Long-pec skeletal muscle myoseptum morphology, abnormal
Long-pec skeletal muscle skeletal muscle myofibril undulate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Genet