ZFIN ID: ZDB-FIG-190723-662
Nittoli et al., 2019 - Characterization of paralogous uncx transcription factor encoding genes in zebrafish. Gene X   2:100011 Full text @ Gene X
Anatomical Terms:
Stage Range: Bud to Long-pec

Fig. 4

Non-somitic expression of uncx4.1. Whole-mount in situ hybridization of uncx4.1 at (A) tailbud stage, (B–D) 18 hours post fertilization (hpf), (E, F) 24 hpf, (G–L) 34 hpf, and (M) 48 hpf. (A, C, F, H, J) Dorsal view, (B, E, G, H, K–M) lateral view, and (D, I) frontal view. (A, C) Anterior to top, (B, E–H, J–M) to left, and (D, I) toward viewer. (A) Early expression in rhombomeres 3–5 (r3, r5) as shown by double in situ labeling with egr2b riboprobe. Expression in telencephalon (Te), olfactory placodes (OP), hypothalamus (Hy), and prospective hindbrain (HB) at (B–D) 18 hpf and (E, F) 24 hpf. Di = diencephalon, HB = hindbrain, Hy = hypothalamus, OP = olfactory placode, Te = telencephalon. (G, H) Expression extends to thalamus at 34 hpf. Ce = cerebellum, Ey = eye, Th = thalamus. (I) Transversal section showing expression in neuronal progenitor cells in the hindbrain at 34 hpf. (J) Arrowheads indicate expression in the hindbrain near exit roots of branchial motor neurons as shown by double in situ labeling with acetylated α-tubulin antibody at 34 hpf. Expression (K) in pharyngeal arches and (L) Corpuscle of Stannius at 34 hpf in lateral view. CS = Corpuscle of Stannius, PA = pharyngeal arches, YE = yolk extension. (M) Expression in central nervous system and (inset) Corpuscle of Stannius at 48 hpf. OT = optic tectum, Yo = yolk.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
uncx4.1 AB standard conditions Bud presumptive rhombomere 2 ISH
Bud presumptive rhombomere 3 ISH
Bud presumptive rhombomere 4 ISH
14-19 somites diencephalon ISH
14-19 somites hindbrain ISH
14-19 somites olfactory placode ISH
14-19 somites telencephalon ISH
Prim-5 diencephalon ISH
Prim-5 hindbrain ISH
Prim-5 hypothalamus ISH
Prim-5 olfactory placode ISH
Prim-5 telencephalon ISH
Prim-15 cerebellum ISH
Prim-15 corpuscles of Stannius ISH
Prim-15 hindbrain ISH
Prim-15 pharyngeal arch ISH
Prim-15 pretectum ISH
Prim-15 pronephric duct ISH
Prim-15 telencephalon ISH
Prim-15 ventral thalamus ISH
Long-pec brain ISH
Long-pec central nervous system ISH
Long-pec corpuscles of Stannius ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
Long-pec thalamus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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