ZFIN ID: ZDB-FIG-190723-1911
Mullapudi et al., 2018 - Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Days 7-13

Figure 1 (A) CRISPR target site in the insulin gene, with PAM sequence highlighted, and the resulting 16 bp deletion allele (below). (A’) Schematic of wild-type Insulin protein and the predicted mutant protein which contains novel sequence (black). (B) Confocal projection images of the pancreatic islet in 96 hpf Tg(ins:GFP) ins +/+ and ins -/- animals immunostained for Insulin (red) and Glucagon (cyan). (C) Free glucose levels in wild-type and mutant animals from 1 to 6 dpf; mean ± SEM, n = 2–4 replicates. (D) Nile Red staining (green) for neutral lipids in 120 hpf wild-type (top) and mutant (bottom) larvae, with yolk lipid content outlined (yellow dots). (E) Genotype distribution from ins ± incross, calculated as the percentage of total animals at each stage; mean ± SEM, n = 32 animals at each stage. (F) Heat map of the proteomic signature of zebrafish ins mutants at 120 hpf compared to signatures from rodent (R) and human (H) diabetic proteome studies. Canonical pathways implicated in most studies are listed first. P-value cut-off set at <0.05. Scale bars: 10 μm (B), 500 μm (D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP insbns102/bns102; zf5Tg standard conditions Day 4 endocrine pancreas IHC
zf5Tg standard conditions Day 4 endocrine pancreas IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-ins IHC insbns102/bns102; zf5Tg standard conditions Day 4 Not Detected endocrine pancreas
IHC zf5Tg standard conditions Day 4 endocrine pancreas
Phenotype Details
Fish Conditions Stage Phenotype
insbns102/bns102 standard conditions Long-pec - Day 6 whole organism glucose increased amount, abnormal
Day 5 yolk lipid increased amount, abnormal
Days 7-13 whole organism decreased life span, abnormal
insbns102/bns102; zf5Tg standard conditions Day 4 endocrine pancreas ab1-ins labeling absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife