Fig. 5

Localization of biotransformation of 7-ethoxyresorufin (ER) in the trunk region of intact zebrafish embryos and larvae at 26 h post-fertilization (hpf) (b,c), 50 hpf (d,e), 74 hpf (f,g), 98 hpf (h,i), 122 hpf (j,k) and 14 d post-fertilization (dpf) (l,m). At 7 hpf (a), qualitative analysis of resorufin formation was performed in the whole embryo. The stage of 9 dpf was excluded from the figure since resorufin formation could not be localized due to ventral position of the larvae. Pictures show one embryo/larva out of six used in the study, i.e., three biological replicates with two embryos/larvae per replicate, for each developmental stage. Figure (a) shows a vegetal pole view of the embryo. In Figure 5 (b–m) lateral views of the anterior and posterior part of the trunk region are shown. The organs in which resorufin had been formed are indicated with a two-letter combination. Since the hatching gland and otic vesicle do not belong to the trunk region, resorufin formation in the respective organs is mentioned separately. S.B.: swim bladder. Scale bar: 200 µm; (b,c): anterior top and dorsal right; (d–m): anterior left and dorsal top.