FIGURE

Fig. 1

ID

ZDB-FIG-190201-31

Publication

Arnandis et al., 2018 - Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Centrosome Amplification Induces Paracrine Invasion

(A) Experimental flowchart.

(B) Left, quantification of invasive structures. Right, normal and invasive 3D acini. White arrowheads indicate invasive protrusions. Scale bar: 20 μM.

(D) Quantification of invasive structures.

(E) Quantification of invasive structures.

(F) Top, schematic representation of mammary organoids isolation and growth. Bottom, non-invasive and invasive mammary organoids. Scale bar: 20 μM.

(G) Quantification of invasive organoids.

(H) Images of zebrafish injected with cells with (+DOX) or without (−DOX) extra centrosomes (left) or co-injected +DOX/−DOX (right).

(I) Incidence of invasive cells in zebrafish embryos. Number of injected fish −DOX = 121; +DOX = 103; and co-injection +/−DOX = 116.

(J) Number of disseminated cells in the zebrafish tail. Error bars represent mean ± SEM.

For all graphics, error bars represent mean ± SD from three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001; ns not significant.

See also Figure S1; Videos S1, S2, S3, and S4; Table S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 47, Arnandis, T., Monteiro, P., Adams, S.D., Bridgeman, V.L., Rajeeve, V., Gadaleta, E., Marzec, J., Chelala, C., Malanchi, I., Cutillas, P.R., Godinho, S.A., Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion, 409-424.e9, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell