Fig. 2

Antitumor effect of anti-angiogenic drugs in the zebrafish embryos. Tumor inhibition effect of three anti-angiogenic drugs in an A549 xenograft zebrafish model. Each compound was evaluated at three different concentrations: bevacizumab (28 ng, 83 ng and 250 ng per embryo), apatinib (0.057 μg/mL, 0.167 μg/mL and 0.5 μg/mL) and endostar (11 ng, 33 ng and 99 ng per embryo). (A) Lateral views of CM-Dil stained A549 cells in 5dpf zebrafish embryos treated with compound for 72 hours. (B) Fluorescence intensity values of A549 cells in zebrafish larvae. (C) Tumor growth inhibition rates is calculated as difference between fluorescence intensities in treated and control groups expressed as percentage of tumor fluorescence intensities in control zebrafish. All quantification data are presented as means ± SEM. P values were calculated with one-way ANOVA followed by Dunnett’s test, **p < 0.01, ***p < 0.001, #p > 0.05 compared to control.