ZFIN ID: ZDB-FIG-181025-18
Ellett et al., 2018 - Macrophages protect Talaromyces marneffei conidia from myeloperoxidase-dependent neutrophil fungicidal activity during infection establishment in vivo. PLoS pathogens   14:e1007063 Full text @ PLoS Pathog.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage Range: Long-pec to Day 6
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Day 4 to Day 6

Fig. 3

Zebrafish mount a Csf3r-dependent granulopoietic response to T. marneffei infection.

(A) Low magnification images of infected and uninfected whole Tg(mpx:EGFP) embryos at 0, 3 and 4 dpi, displaying the profound infection-driven increase in EGFP-expressing neutrophil numbers. (B, C) Neutrophil (B) and macrophage (C) population expansion in infected versus uninfected embryos from 0–4 dpi. These data derive from enumerating neutrophil and macrophage numbers in the same embryos, of genotype Tg(mpx:EGFP/mpeg1:Gal4FF/UAS-E1b:Eco.nfsB-mCherry). Data displayed are mean±SD, n = 4 embryos/group. These data replicate other data derived from separately scoring these leukocyte populations in different embryos, in n≥2 independent other experiments. (D) Neutrophil population sizes at 4 dpi represented by Neutrophil Units in embryos injected with antisense morpholino oligonucleotides targeted to knockdown csf3-receptor (csf3r-MO) or interleukin-6 receptor (il6ra-MO and gp130-MO) Data are mean±SEM, n = 10 embryos/group/experiment, n≥3 experiments. Intragroup +/- infection p-values from 2-tailed unpaired t-test; p-values from intergroup comparisons involving csf3r-MO from 1-tailed unpaired t-test, given the a priori expectation of a reduction in neutrophil values in csf3r-deficiency [65, 95] (E) Kaplan-Meier life table analysis for the treatment groups shown in (C). Numbers/group (uninfected/infected): Control MO, 474/326; csf3r-MO, 443/233; il6ra-MO, 462/330; gp130-MO, 465/313. P-values from Gerhan-Breslow-Wilcoxon Test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP gl27Tg control Long-pec neutrophil IFL
Day 5 neutrophil IFL
Day 6 neutrophil IFL
gl27Tg fungal treatment by injection: Talaromyces marneffei Long-pec neutrophil IFL
Day 5 neutrophil IFL
Day 6 neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-il6r fungal treatment by injection: Talaromyces marneffei Day 6 neutrophil increased amount, abnormal
Day 6 whole organism life span, normal
AB + MO1-il6st fungal treatment by injection: Talaromyces marneffei Day 6 neutrophil increased amount, abnormal
Day 6 whole organism life span, normal
AB + MO3-csf3r control Day 6 neutrophil decreased amount, abnormal
AB + MO3-csf3r fungal treatment by injection: Talaromyces marneffei Day 5 - Day 6 whole organism decreased life span, abnormal
Day 6 neutrophil increased amount, ameliorated
gl27Tg fungal treatment by injection: Talaromyces marneffei Day 4 neutrophil increased amount, abnormal
Day 5 macrophage increased amount, abnormal
Day 5 neutrophil increased amount, abnormal
Day 6 macrophage increased amount, abnormal
Day 6 neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS pathogens for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Pathog.