Effects of Ponatinib on vascular development of zebrafish larvae. (A) Fluorescent images of ISV and SIV in the zebrafish embryos incubated with 0.1% DMSO (Ctrl: vehicle-control), VRI (5 μM), and Ponatinib (1, 3 and 10 μM) for 48 h. VRI was used as the positive control. Red arrow: normal ISV. (B) Number of normal ISV with full-length formation. (C) Quantification of SIV area as a percentage of the control group. The total SIV area was determined by using the NIH image J program. (D) Formation of selected ISV (NO. 4, 7, and 10). All values are presented as means ± SD (n = 12). *P < 0.05 vs. control group, and ***P < 0.005 vs. control group.