FIGURE

Fig. 1

ID

ZDB-FIG-180823-1

Publication

Guzzolino et al., 2018 - Post-transcriptional Modulation of Sphingosine-1-Phosphate Receptor 1 by miR-19a Affects Cardiovascular Development in Zebrafish

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Fig. 1

s1pr1 overexpression affects vascular development. (A) Confocal microscopy of representative 48 hpf Tg(kdrl:EGFP)^s843 embryos showing vascular defects of different severity. The Tg(kdrl:EGFP)^s843 embryos were injected with in vitro transcribed and capped mRNA of the full-length (CDS+UTR) or the CDS sequence of s1pr1 at the reported doses (pg). The total numbers of embryos analyzed were as follows: 300 pg RFP CDS injected n = 20; 150 pg s1pr1 CDS injected n = 22; 300 pg s1pr1 CDS injected n = 38; 300 pg s1pr1 CDS+UTR injected n = 15. Red arrows indicate examples of shorter and incomplete ISVs. Scale bar = 500 μm (zoom 100 μm). (B) Blinded quantification of the abnormal vascular phenotypes showed in (A). **P < 0.001, ***P < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol