ZFIN ID: ZDB-FIG-180730-39
Juan et al., 2018 - Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry. Nature communications   9:1942 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 1

myo1D controls zebrafish left–right asymmetry. a Transgene-mediated expression of zebrafish myo1D (zmyo1D) restores the laterality of genitalia rotation in Drosophila myo1D (dmyo1D) mutant or dmyo1D RNAi flies. Lines 3 and 6 are two independent transgenic insertions. be Zebrafish MZ myo1D mutants present defects in leftward cardiac jogging. bd Dorsal views of the cmlc2-expressing heart primordium at 26 hpf, anterior up. e Quantification of cardiac jogging defects in maternal (M) and maternal zygotic (MZ) myo1Dtj16b or myo1Dtj16c mutants. fi Cardiac looping is affected in MZ myo1D mutants. fh Frontal views of the cmlc2-expressing heart at 48 hpf, dorsal up. i Quantification of cardiac looping defects in M myo1Dtj16b and MZ myo1Dtj16b or MZ myo1Dtj16c mutants. jm MZ myo1D mutants present defects in the leftward looping of the gut and the asymmetric development of the liver (black arrows) and pancreas (white arrows). jl Dorsal views of foxa1-expressing visceral organs at 48 hpf, anterior up. n, o Overexpression of zebrafish myo1Cb RNA impairs cardiac jogging (n) and looping (o). MZ myo1G single mutants present laterality defects (n, o). MZ myo1D; MZ myo1G double mutants or MZ myo1D mutants injected with myo1Cb RNA present an increased frequency of defects compared to MZ myo1D mutants (n, o). Scale bars: 30 µm

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxa1 WT standard conditions Long-pec digestive system ISH
myo1dnce16b/nce16b standard conditions Long-pec digestive system ISH
myl7 WT standard conditions Prim-5 primitive heart tube ISH
Long-pec heart ISH
myo1dnce16b/nce16b standard conditions Prim-5 primitive heart tube ISH
Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
myo1dnce16b/nce16b standard conditions Prim-5 determination of heart left/right asymmetry decreased process quality, abnormal
Prim-5 heart tube heart jogging decreased process quality, abnormal
Prim-5 heart tube mislocalised, abnormal
Long-pec determination of heart left/right asymmetry decreased process quality, abnormal
Long-pec determination of liver left/right asymmetry process quality, abnormal
Long-pec determination of pancreatic left/right asymmetry process quality, abnormal
Long-pec gut determination of digestive tract left/right asymmetry process quality, abnormal
Long-pec heart heart looping decreased process quality, abnormal
Long-pec heart mislocalised, abnormal
myo1dnce16b/nce16b ; myo1gnce18b/nce18b standard conditions Prim-5 determination of heart left/right asymmetry decreased process quality, exacerbated
Prim-5 heart tube heart jogging decreased process quality, exacerbated
Prim-5 heart tube mislocalised, exacerbated
Long-pec determination of heart left/right asymmetry decreased process quality, exacerbated
Long-pec heart heart looping decreased process quality, exacerbated
Long-pec heart mislocalised, exacerbated
myo1dnce16c/nce16c standard conditions Prim-5 determination of heart left/right asymmetry decreased process quality, abnormal
Prim-5 heart tube heart jogging decreased process quality, abnormal
Prim-5 heart tube mislocalised, abnormal
Long-pec determination of heart left/right asymmetry decreased process quality, abnormal
Long-pec heart heart looping decreased process quality, abnormal
Long-pec heart mislocalised, abnormal
myo1gsa17276/sa17276 standard conditions Prim-5 determination of heart left/right asymmetry decreased process quality, abnormal
Prim-5 heart tube heart jogging decreased process quality, abnormal
Prim-5 heart tube mislocalised, abnormal
Long-pec determination of heart left/right asymmetry decreased process quality, abnormal
Long-pec heart heart looping decreased process quality, abnormal
Long-pec heart mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.