ZFIN ID: ZDB-FIG-180713-3
Almeida et al., 2018 - Myelination of Neuronal Cell Bodies when Myelin Supply Exceeds Axonal Demand. Current biology : CB   28(8):1296-1305.e5 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Day 4 to Days 7-13
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Day 4 to Days 7-13

Fig. 1

Oligodendrocytes in Excess of Target Axons Ectopically Myelinate Neuronal Cell Bodies in kif1bp Mutants

(A) Double myelin and oligodendrocyte reporter showing oligodendrocytes (asterisks) and ectopic profiles (arrowheads) in the spinal cord (4 dpf).

(B) Oligodendrocyte number (per 100 μm length of spinal cord) is normal in the anterior but reduced in the posterior spinal cord of kif1bp mutants (p = 0.688 anterior; p = 0.0001 posterior; n = 11 wild-type [WT] and n = 11 mutants; t test).

(C) Profile number is increased in mutants (p = 0.018 anterior; p = 0.011 posterior; n = 11 WT and n = 11 mutants; t test).

(D) Double myelin and neuronal reporter showing mCherry+ neurons enwrapped by EGFP+ myelin (arrowheads) in 4 dpf mutants. Asterisk, oligodendrocyte cell body.

(E) Time course of double oligodendrocyte and myelin reporter in kif1bp mutant. Asterisks denote new oligodendrocytes.

(F) Transmission electron microscopy (TEM) of a typical unmyelinated cell body and four myelinated cell bodies in 6 dpf mutants.

(G) 4 dpf anterior spinal cord of oligodendrocyte and myelin reporter showing that increased ectopic profiles (arrows) in mutants are rescued by presence of additional Mauthner axons (red brackets) when injected with notch1a morpholino (MO).

(H) Oligodendrocyte number (normalized to control average) is normal in all conditions (p[kif1bp-WT] = 0.267; p[WT+notch1a MO-WT] = 0.483; p[kif1bp+notch1aMO-WT] = 0.374; p[kif1bp+notch1aMO-kif1bp] = 0.071; n = 23 WT; n = 18 kif1bp; n = 22 WT+notch1a MO; n = 19 kif1bp+notch1a MO; t tests).

(I) Myelinated cell body number (normalized to control average) is increased in mutants (p[WT-kif1bp] < 0.0001, n = 23 WT and n = 18 kif1bp anterior; p[WT-kif1bp] = 0.0019, n = 14 WT and n = 6 kif1bp posterior; t tests) and is rescued by additional Mauthner axons in anterior (p[kif1bp+notch1a MO-kif1bp] = 0.0008, n = 18 kif1bp and n = 19 kif1bp+notch1a MO; t test), but not posterior, spinal cord of mutants (p[kif1bp+notch1a MO-kif1bp] = 0.359, n = 6 kif1bp and n = 5 kif1bp+notch1a MO; t test).

All graphs display mean and SD. OL, oligodendrocyte. The scale bars represent 10 μm (A, E, and G), 5 μm (D), 0.5 μm (F, whole cell), and 0.1 μm (F, myelin detail). See also Movies S1 and S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ue1Tg ; ue2Tg standard conditions Day 4 oligodendrocyte cytoplasm IFL
Day 4 oligodendrocyte myelin sheath IFL
Day 4 oligodendrocyte plasma membrane IFL
ue2Tg ; kifbpst23/st23 ; ue3Tg standard conditions Day 4 oligodendrocyte myelin sheath IFL
Day 4 oligodendrocyte plasma membrane IFL
ue2Tg ; ue1Tg ; kifbpst23/st23 standard conditions Day 4 oligodendrocyte cytoplasm IFL
Day 4 oligodendrocyte myelin sheath IFL
Day 4 oligodendrocyte plasma membrane IFL
Days 7-13 oligodendrocyte cytoplasm IFL
Days 7-13 oligodendrocyte myelin sheath IFL
Days 7-13 oligodendrocyte plasma membrane IFL
ue2Tg ; ue1Tg ; kifbpst23/st23 + MO5-notch1a standard conditions Day 4 oligodendrocyte cytoplasm IFL
Day 4 oligodendrocyte myelin sheath IFL
Day 4 oligodendrocyte plasma membrane IFL
mCherry ue2Tg ; kifbpst23/st23 ; ue3Tg standard conditions Day 4 neuron cytoplasm IFL
Day 4 spinal cord neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kifbpst23/st23 standard conditions Day 6 oligodendrocyte myelin sheath abutting neuron cell body, abnormal
ue1Tg ; ue2Tg + MO5-notch1a standard conditions Day 4 hindbrain has extra parts of type Mauthner neuron, abnormal
Day 4 oligodendrocyte myelin sheath abutting neuron axon, normal
Day 4 oligodendrocyte myelin sheath separated from neuron cell body, normal
Day 4 spinal cord anterior region has extra parts of type Mauthner neuron axon, abnormal
Day 4 spinal cord anterior region has normal numbers of parts of type oligodendrocyte, normal
ue2Tg ; kifbpst23/st23 ; ue3Tg standard conditions Day 4 oligodendrocyte myelin sheath abutting neuron cell body, abnormal
Day 4 spinal cord anterior region has normal numbers of parts of type oligodendrocyte, normal
ue2Tg ; ue1Tg ; kifbpst23/st23 standard conditions Day 4 oligodendrocyte myelin sheath abutting neuron cell body, abnormal
Day 4 reticulospinal neuron axon extension decreased occurrence, abnormal
Day 4 spinal cord anterior region has fewer parts of type reticulospinal neuron axon, abnormal
Day 4 spinal cord anterior region has normal numbers of parts of type oligodendrocyte, normal
Day 4 spinal cord posterior region has fewer parts of type oligodendrocyte, abnormal
Day 4 spinal cord posterior region has fewer parts of type reticulospinal neuron axon, abnormal
Days 7-13 oligodendrocyte myelin sheath abutting neuron cell body, abnormal
Days 7-13 reticulospinal neuron axon extension decreased occurrence, abnormal
ue2Tg ; ue1Tg ; kifbpst23/st23 + MO5-notch1a standard conditions Day 4 hindbrain has extra parts of type Mauthner neuron, abnormal
Day 4 oligodendrocyte myelin sheath abutting neuron axon, normal
Day 4 oligodendrocyte myelin sheath distal to neuron cell body, ameliorated
Day 4 oligodendrocyte myelin sheath separated from neuron cell body, ameliorated
Day 4 spinal cord anterior region has extra parts of type Mauthner neuron axon, abnormal
Day 4 spinal cord anterior region has normal numbers of parts of type oligodendrocyte, normal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.