Fig. 5

Hsp90aa1.2 function is important for cell cycle progression. (A) Schematic overview of the Fucci system. (B) Schematic representation of the Fucci system in PGCs in which Hsp90aa1.2 activity was reduced. (C) Representative images of migrating PGCs expressing the Fucci cell cycle markers in control embryos (left panels) or in embryos knocked down for Hsp90aa1.2 function (right panels). (D) Higher proportion of abnormal simultaneous expression of Geminin and Cdt1 in PGCs knocked down for Hsp90aa1.2. The effect is reversed by introduction of MO-resistant hsp90aa1.2-nanos3 3’UTR RNA. (E) 17-DMAG treatment enhances the proportion of PGCs exhibiting simultaneous expression of Geminin and Cdt1. (F) Higher proportion of abnormal simultaneous expression of Geminin and Cdt1 in PGCs within embryos carrying mutations in the hsp90aa1.2 locus (two experiments with the hsp90aa1.2d5 and one with the hsp90aa1.2d34 allele) as compared with PGCs in mutant embryos injected with wild-type hsp90aa1.2 RNA. (G) The fraction of dividing PGCs within the time frame of 6–12 hpf and of 12–18 hpf. The analysis was performed on wild-type embryos injected with control RNA, mutant embryos injected with control RNA and mutant embryos supplemented with hsp90aa1.2 RNA. The experiments were performed using the hsp90aa1.2d34 and the hsp90aa1.2d5 alleles. n is the number of PGCs analyzed.