ZFIN ID: ZDB-FIG-180706-59
Pfeiffer et al., 2018 - Rapid progression through the cell cycle ensures efficient migration of primordial germ cells - the role of Hsp90. Developmental Biology   436(2):84-93 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 75%-epiboly

Fig. 4

Hsp90aa1.2 function is important for microtubule organization. (A) In control PGCs, the single MTOC is positioned at the back of the migrating cell (n = 31 cells, 4 cells showing two MTOCs (13%); upper panels), while in embryos in which Hsp90aa1.2 is knocked down, a large proportion of the cells show a duplicated MTOC (n = 22 cells, 11 cells with two MTOCs (50%); lower panels). (B) In embryos mutated for hsp90aa1.2, duplicated MTOCs are found in the migrating PGCs (n = 41 cells, 22 cells with two MTOCs (52%); lower panels), a phenomenon reversed by introduction of RNA encoding for Hsp90aa1.2 in PGCs by fusing the open reading frame of the RNA to the 3’UTR of the nanos3 gene (n = 51 cells, 9 cells with two MTOCs (18%); upper panels). The experiments were performed using the hsp90aa1.2d34 and the hsp90aa1.2d5 alleles, as well as in embryos harboring both alleles.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-hsp90aa1.1,hsp90aa1.2 control Shield - 75%-epiboly primordial germ cell has extra parts of type primordial germ cell microtubule organizing center, abnormal
Shield - 75%-epiboly primordial germ cell microtubule organizing center organization decreased process quality, abnormal
hsp90aa1.2mu10/mu10(AB) control Shield - 75%-epiboly primordial germ cell has extra parts of type primordial germ cell microtubule organizing center, abnormal
Shield - 75%-epiboly primordial germ cell microtubule organizing center organization decreased process quality, abnormal
hsp90aa1.2mu9/mu9(AB) control Shield - 75%-epiboly primordial germ cell has extra parts of type primordial germ cell microtubule organizing center, abnormal
Shield - 75%-epiboly primordial germ cell microtubule organizing center organization decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 436(2), Pfeiffer, J., Tarbashevich, K., Bandemer, J., Palm, T., Raz, E., Rapid progression through the cell cycle ensures efficient migration of primordial germ cells - the role of Hsp90, 84-93, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.