ZFIN ID: ZDB-FIG-180703-33
Campbell et al., 2018 - Directing Nanoparticle Biodistribution Through Evasion and Exploitation of Stab2-Dependent Nanoparticle Uptake. ACS nano   12(3):2138-2150 Full text @ ACS Nano
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Adult

Fig. 3

stab2 is required for anionic liposome uptake by SECs. (a, b) Tissue level view of DOPG (a) and DSPC (b) liposome distribution at 1 hpi in control and dextran sulfate injected embryos, with quantification of liposome levels associated with venous vs arterial endothelial cells based on rhodamine fluorescence intensity associated with CV vs DA. (c) stab2 domain structure predicted to be expressed from the wild-type stab2 and the stab2ibl2 allele. (d) Whole-embryo view of flt1:RFP, flt4:YFP double transgenic embryos at 5 dpf to visualize blood vascular and lymphatic development. No defects were identified during (lymph)angiogenesis and vascular patterning in stab2ibl2 homozygous embryos compared to sibling controls. (e) Fertile adult females (stab2ibl2 homozygous and sibling controls) at 3 months post-fertilization. (f–k) Tissue level view of fluoHA (f) and DOPG (g), DSPC (h), AmBisome (i), EndoTAG-1 (j), and Myocet (k) liposome distribution at 1 hpi in stab2ibl2 and sibling control embryos, with quantification of liposome levels associated with venous vs arterial endothelial cells based on rhodamine fluorescence intensity associated with CV vs DA. (a, b, f–k) Bar height represents median values, dots represent individual data points, and brackets indicate significantly different values (*: p < 0.05, **: p < 0.01, ***: p < 0.001, N.S.: not significant) based on Mann–Whitney test. n = 6–10 per group (in two experiments).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine stab2ibl2/ibl2; hu5333Tg; hu7135Tg control Day 5 lymph vasculature IFL
RFP stab2ibl2/ibl2; hu5333Tg; hu7135Tg control Day 5 blood vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
stab2ibl2/ibl2 control Adult whole organism fertility, normal
Adult whole organism morphology, normal
stab2ibl2/ibl2 chemical treatment by injection: carbon black nanoparticle, chemical treatment by injection: lipid micelle Long-pec hepatic sinusoid carbon black nanoparticle decreased amount, abnormal
Long-pec hepatic sinusoid import into cell decreased occurrence, abnormal
stab2ibl2/ibl2; hu5333Tg; hu7135Tg control Day 5 blood vessel development process quality, normal
Day 5 lymph vessel development process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal ACS nano for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ ACS Nano