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ZFIN ID: ZDB-FIG-180425-29
Madakashira et al., 2017 - Variant Histone H2afv Reprograms DNA Methylation During Early Zebrafish Development. Epigenetics   12(9):811-824 Full text @ Epigenetics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 64-cell to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 1

H2afv expression successfully depleted by morpholino (MO) injection (A) RT-PCR using primers that detect both h2afva and h2afvb demonstrates high mRNA levels of expression across all stages of embryonic zebrafish development. (B) Whole mount immunofluorescence detects H2afv protein expression across stages of zebrafish embryonic development. Representative images from at least 5 embryos examined from 2 separate clutches are shown. (C) Western blot analysis demonstrates depletion of H2afv expression at 24 hpf in lysates from varying concentrations of h2afv MO injected embryos compared with control (uninjected) embryos. h2afv morphants (0.67 ng) were used for the rest of the study (marked*). Representative image of 2 different blots are shown. (D) Kaplan-Meier curve illustrates increased mortality of h2afv morphants compared with control (uninjected) embryos across 96 h post fertilization (hpf). MO injected at 3.3, 0.67 and 0.34 ng per embryo, n = number of clutches and number of total embryos analyzed per condition in parenthesis.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-h2afva IHC WT standard conditions 64-cell whole organism
IHC High whole organism
IHC Bud whole organism
IHC 14-19 somites whole organism
WB WT + MO1-h2afvb standard conditions Prim-5 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-h2afvb standard conditions Prim-5 whole organism Ab1-h2afva labeling decreased amount, abnormal
Prim-5 - Day 4 whole organism decreased life span, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Epigenetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Epigenetics