FIGURE

Fig. 6

ID

ZDB-FIG-180420-32

Publication

Dogra et al., 2017 - Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

CM proliferation is inversely regulated by Smad2 and Smad3. a Experimental setup of SIS3 treatment, followed by EdU treatment and fixation. b, c Tg(myl7:nlsDsRedExpress) hearts of untreated control and 3 µM SIS3-treated larvae at 120 hpf; α-DsRed (red), EdU (green). White arrowheads point to proliferating CMs (EdU⁺/DsRed⁺). d Quantification of CM proliferation in untreated control (n = 4) and 3 µM SIS3-treated (n = 5) ventricles at 120 hpf. e Experimental setup of injections, followed by EdU treatment and fixation. f–i Tg(myl7:nlsDsRedExpress) hearts of myl7:H2B-EGFP, myl7:H2B-EGFP-2A-caSmad2, myl7:H2B-EGFP-2A-caSmad3a and myl7:H2B-EGFP-2A-caSmad3b injected larvae at 120 hpf; α-DsRed (red), α-GFP (blue), EdU (green). White arrowheads point to proliferating CMs (EdU⁺/DsRed⁺/GFP ⁺). j Quantification of CM proliferation in myl7:H2B-EGFP (n = 10), caSmad2 (n = 10), caSmad3a (n = 10), and caSmad3b (n = 10) injected ventricles at 120 hpf. All cell counts were performed on non-overlapping confocal planes (thickness, 1 µm) (data are mean ± s.e.m., *P ≤ 0.05 and ***P ≤ 0.001—Student’s t test, two-tailed). Scale bars, 20 µm. vent., ventricle; atr., atrium

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hsc4Tg
Condition:	chemical treatment by environment: SIS3
Observed In:	heart cardiac muscle cell proliferation
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.