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ZFIN ID: ZDB-FIG-180420-31
Dogra et al., 2017 - Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair. Nature communications   8:1902 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 5

mstnb and inhbaa inversely regulate the activities of Smad2 and Smad3 response elements, as well as Smad3 phosphorylation. a RT-qPCR analysis for relative EGFP mRNA expression in 48 hpf Tg(ARE:EGFP) embryos injected with mstnb, inhbaa, mstnb-2A-H2B-mcherry, and inhbaa-2A-H2B-mcherry mRNA compared to RFP mRNA injected (n = 2 × 10 embryos assessed as 2 biological and 2 technical replicates). b RT-qPCR analysis for relative EGFP mRNA expression in 48 hpf Tg(12XSBE:EGFP) embryos injected with mstnb, inhbaa, mstnb-2A-H2B-mcherry, and inhbaa-2A-H2B-mcherry mRNA compared to RFP mRNA injected (n = 2 × 10 embryos assessed as two biological and two technical replicates). c, d Sections of wild-type sibling and mstnb OE cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). White dotted regions delineate the injured area. White arrowheads point to pSmad3+ CMs near the injured area. e Quantification of pSmad3+ CMs in wild-type sibling (n = 4) and mstnb OE (n = 5) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. f, g Sections of wild-type sibling and inhbaa OE cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). h Quantification of pSmad3+ CMs in wild-type sibling (n = 3) and inhbaa OE (n = 4) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. i, j Sections of mstnb +/+ and mstnb −/− cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). k Quantification of pSmad3+ CMs in mstnb +/+ (n = 4) and mstnb −/− (n = 4) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. l, m Sections of inhbaa +/+ and inhbaa −/− cryoinjured hearts at 14 dpci; pSmad3 (green), α-MF-20 (red), DAPI (blue). n Quantification of pSmad3+ CMs in inhbaa +/+ (n = 4) and inhbaa −/− (n = 5) cryoinjured hearts in the 100 µm region adjacent to the injured area at 14 dpci. All cell counts were performed on three sections from each heart. Each data point on dot plot represents one heart (data are mean ± s.e.m., *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001—Student’s t test, two-tailed). Scale bars, 100 µm

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT cold damage: heart Adult myocardium
IHC bns145Tg cold damage: heart Adult myocardium
IHC bns146Tg cold damage: heart Adult myocardium
IHC inhbaabns37/bns37 cold damage: heart Adult myocardium
IHC mstnbbns5/bns5 cold damage: heart Adult myocardium
Ab2-smad3 IHC WT cold damage: heart Adult cardiac muscle myoblast
IHC bns145Tg cold damage: heart Adult cardiac muscle myoblast
IHC bns146Tg cold damage: heart Adult cardiac muscle myoblast
IHC inhbaabns37/bns37 cold damage: heart Adult cardiac muscle myoblast
IHC mstnbbns5/bns5 cold damage: heart Adult cardiac muscle myoblast
Phenotype Details
Fish Conditions Stage Phenotype
bns145Tg cold damage: heart Adult cardiac muscle myoblast ab2-smad3 labeling decreased distribution, abnormal
bns145Tg cold damage: heart Adult heart cardiac muscle cell proliferation decreased occurrence, abnormal
bns146Tg cold damage: heart Adult cardiac muscle myoblast ab2-smad3 labeling increased distribution, abnormal
Adult heart cardiac muscle cell proliferation increased occurrence, abnormal
inhbaabns37/bns37 cold damage: heart Adult cardiac muscle myoblast ab2-smad3 labeling decreased distribution, abnormal
Adult heart cardiac muscle cell proliferation decreased occurrence, abnormal
mstnbbns5/bns5 cold damage: heart Adult cardiac muscle myoblast ab2-smad3 labeling increased distribution, abnormal
Adult heart cardiac muscle cell proliferation increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.