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ZIRC
ZFIN ID: ZDB-FIG-180322-7
Mojib et al., 2017 - Zebrafish aversive taste co-receptor is expressed in both chemo- and mechanosensory cells and plays a role in lateral line development. Scientific Reports   7:13475 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Days 7-13

Fig. 3

RL-TGR is expressed in the neuromasts. (a) Confocal images of a single neuromast of the posterior lateral line system of wild type embryo at 4 dpf (lateral view). RL-TGR (green) staining signal was detected in hair cells (hc), support cells (sc), and mantle cells (mc) of the neuromast. The image on the bottom right represents the scheme of a neuromast illustrating different cells and their organization (modified after Ghysen and Dambly-Chaudiere, Current Opinion in Neurobiology, 2004)33. (b) Confocal images of a neuromast of wild type embryo (dorso-lateral view) at 7 dpf stained with RL-TGR (green, expression in the hair cells), acetylated tubulin (blue, expression in the cilia of the hair cells), and neuromast marker Prox1 (magenta, expression in the hair cells, supporting cells, and mantle cells). Colocalization of RL-TGR and acetylated tubulin suggests that RL-TGR is highly expressed in both kinocilia and cell bodies of the mechanosensory hair cells. RL-TGR colocalized with Prox1 in the stereocilia of the hair cells and supporting cells surrounding the neuromast. (c) 3D maximum intensity projection images of a neuromast of wild type embryo (lateral view) at 7 dpf stained with RL-TGR (green, expression in the hair cells), parvalbumin (PVALB) (red, expression in the hair cells). nā€‰>ā€‰50 embryos; schematic diagram of the 7 dpf larvae shows the location of different images; scale bars are shown in the images.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rltgr Ab1-rltgr AB/TU standard conditions Day 4 neuromast hair cell IHC
Ab1-rltgr Day 4 neuromast mantle cell IHC
Ab1-rltgr Day 4 neuromast support cell IHC
Ab1-rltgr Days 7-13 mechanoreceptor cell kinocilium IHC
Ab1-rltgr Days 7-13 neuromast hair cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb IHC AB/TU standard conditions Days 7-13 neuromast hair cell
Ab1-tuba IHC AB/TU standard conditions Days 7-13 mechanoreceptor cell kinocilium
Ab4-prox1 IHC AB/TU standard conditions Days 7-13 neuromast hair cell
IHC Days 7-13 neuromast mantle cell
IHC Days 7-13 neuromast support cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.