ZFIN ID: ZDB-FIG-180321-20
Berger et al., 2017 - sept8a and sept8b mRNA expression in the developing and adult zebrafish. Gene expression patterns : GEP   25-26:8-21 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 1 Exression of sept8aand sept8b in embryonic and early larvae zebrafish revealed by RNA in situ hybridization.


1–4 dpf zebrafish are shown in dorsal and lateral views. Head structures are enlarged in the right-hand panels. A – H: sept8a expression was primarily detectable in ventral body regions at 1 dpf and additionally in single brain structures at 2 dpf. At 3 dpf and 4 dpf sept8a expression was detectable in the gill arches and the brain. I – P: sept8b transcripts were also detectable in ventral regions as well as in cells above the yolk extension (black arrowheads) at 2 dpf. sept8b showed a specific distribution in the midbrain and hindbrain of 2–4 dpf zebrafish. See list for abbreviations. The boxed area in J is enlarged in the inset image. The dotted line in K marks the level of the transverse section shown in the inset image. Scale bars: Whole mounts: 500μm Head structures: 200μm Insets: 50μm.

showed a specific distribution in the midbrain and hindbrain of 2–4 dpf zebrafish. See list for abbreviations. The boxed area in J is enlarged in the inset image. The dotted line in K marks the level of the transverse section shown in the inset image. Scale bars: Whole mounts: 500μ Head structures: 200μ; Insets: 50μ.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sept8a mitfaw2/w2; mpv17b18/b18 control Prim-5 brain ISH
Prim-5 cerebellum ISH
Prim-5 hindbrain ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 solid lens vesicle ISH
Long-pec cerebellum ISH
Long-pec hindbrain ISH
Long-pec lens ISH
Long-pec midbrain hindbrain boundary ISH
Long-pec optic tectum ISH
Protruding-mouth forebrain ISH
Protruding-mouth hindbrain ISH
Protruding-mouth midbrain ISH
Protruding-mouth midbrain hindbrain boundary ISH
Protruding-mouth pharyngeal arch 3-7 ISH
Protruding-mouth retina ISH
Protruding-mouth retinal inner nuclear layer ISH
Day 4 forebrain ISH
Day 4 hindbrain ISH
Day 4 midbrain ISH
Day 4 midbrain hindbrain boundary ISH
Day 4 pharyngeal arch 3-7 ISH
Day 4 retina ISH
Day 4 retinal inner nuclear layer ISH
sept8b mitfaw2/w2; mpv17b18/b18 control Prim-5 brain ISH
Prim-5 cerebellum ISH
Prim-5 hindbrain ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 solid lens vesicle ISH
Long-pec cerebellum ISH
Long-pec eye ISH
Long-pec forebrain ISH
Long-pec hindbrain ISH
Long-pec intestine ISH
Long-pec midbrain ISH
Long-pec midbrain hindbrain boundary ISH
Long-pec optic tectum ISH
Long-pec spinal cord ISH
Protruding-mouth forebrain ISH
Protruding-mouth hindbrain ISH
Protruding-mouth midbrain ISH
Protruding-mouth optic tectum ISH
Protruding-mouth pharyngeal arch 3-7 ISH
Protruding-mouth retina ISH
Protruding-mouth retinal inner nuclear layer ISH
Day 4 forebrain ISH
Day 4 hindbrain ISH
Day 4 midbrain ISH
Day 4 optic tectum ISH
Day 4 pharyngeal arch 3-7 ISH
Day 4 retina ISH
Day 4 retinal inner nuclear layer ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 25-26, Berger, C., Helmprobst, F., Chapouton, P., Lillesaar, C., Stigloher, C., sept8a and sept8b mRNA expression in the developing and adult zebrafish, 8-21, Copyright (2017) with permission from Elsevier. Full text @ Gene Expr. Patterns