Fig. 5

mRNA and protein analysis of two isoforms in mouse. A. Schematic map of primers in the RT-PCR and Q-PCR analysis. B. RT-PCR images of two transcripts among different tissues. C and D. Q-PCR analysis results of two transcripts in one representative experiment. Most tissues had more of long transcript than the short one. But the brain had a reverse ratio, and the testis and heart almost had the equal amounts of two transcripts. Osteoclasts were the cells that only expressed long transcript among the tissues or cells we detected. Error bars labeled the highest and lowest RQ value. E. Western blot analysis showed the different ratios of two isoforms in brain and kidney. F. Immunohistochemical staining showed the ATP6V1H locations in kidney and brain.