ZFIN ID: ZDB-FIG-170809-4
Mesureur et al., 2017 - Macrophages, but not neutrophils, are critical for proliferation of Burkholderia cenocepacia and ensuing host-damaging inflammation. PLoS pathogens   13:e1006437 Full text @ PLoS Pathog.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Day 4

Fig. 1

Macrophages are critical for virulence of B. cenocepacia.

(A,B) Embryo survival (average inoculum 17 CFU, representative experiment) (A) and bacterial burden (total of 3 experiments) over time (B) of control (black) and pu.1 knockdown embryos (red) injected iv with B. cenocepacia K56-2. (C,D) Representative fluorescence overlay images of an mpeg1:mCherry control and mpeg1:mCherry pu.1 knockdown embryo at 30 min and 24 h after injection with ~40 CFU B. cenocepacia K56-2 (blue). See also S2 Fig. (C) mCherry-positive macrophages (red) colocalise with K56-2 at 30 mpi, and are no longer detected at 24 hpi (insets show magnification). (D) mCherry-positive macrophages are absent in knockdown embryos at 30 mpi and start to re-appear at 24 hpi (insets show magnification). Scale bars, 100 μm. (E,F) Embryo survival (average inoculum 28 CFU, representative experiment) (E) and corresponding bacterial burden (n = 10 per group per time point). (F) of mpeg1/umn+ embryos, untreated or treated with 5mM Mtz or 0.2% DMSO, and mpeg1/umnembryos treated with 5mM Mtz iv injected with B. cenocepacia K56-2. (B,F) Geometric means with each data point representing an individual embryo. Dead embryos marked as black open circles (not recorded for 5 embryos in (B), and in (F)). (A, B, E, F) * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; **** p ≤ 0.0001. ns: not significant. See materials and methods for statistical tests. See also S1 Fig and S3 Fig.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mCherry ump2Tg bacterial treatment by injection: Burkholderia cenocepacia Prim-15 macrophage IFL
Long-pec Not Detected macrophage IFL
ump2Tg + MO3-spi1b bacterial treatment by injection: Burkholderia cenocepacia Prim-15 Not Detected macrophage IFL
Long-pec Not Detected macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB bacterial treatment by injection: Burkholderia cenocepacia Prim-15 - Day 4 whole organism decreased life span, abnormal
AB + MO3-spi1b bacterial treatment by injection: Burkholderia cenocepacia Prim-15 - Day 4 whole organism decreased life span, ameliorated
gl24Tg; i149Tg; i186Tg; s1999tTg (AB) bacterial treatment by injection: Burkholderia cenocepacia Prim-15 - Day 4 whole organism decreased life span, abnormal
gl24Tg; i149Tg; i186Tg; s1999tTg (AB) bacterial treatment by injection: Burkholderia cenocepacia, chemical ablation: macrophage, chemical treatment by environment: metronidazole Prim-15 - Day 4 whole organism decreased life span, ameliorated
ump2Tg bacterial treatment by injection: Burkholderia cenocepacia Long-pec macrophage absent, abnormal
Long-pec macrophage decreased amount, abnormal
Long-pec macrophage mCherry expression absent, abnormal
ump2Tg + MO3-spi1b bacterial treatment by injection: Burkholderia cenocepacia Prim-15 macrophage absent, abnormal
Prim-15 macrophage decreased amount, abnormal
Prim-15 macrophage mCherry expression absent, abnormal
Long-pec macrophage absent, abnormal
Long-pec macrophage decreased amount, abnormal
Long-pec macrophage mCherry expression absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.