FIGURE

Fig. S7

ID

ZDB-FIG-170615-17

Publication

Liu et al., 2017 - Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action

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Fig. S7

a) Four different levels of pS6 signal were observed in zebrafish larvae and used for quantification. b) Torin1 treatment abolishes low [Ca²⁺]-induced pAkt signaling. 72 hpf wild type larvae were transferred to 0.2 or 0.001 mM [Ca²⁺] solution containing DMSO or Torin1 (1μM). After 8h, they were subjected to immunostaining using an anti-phospho-Akt (S473) antibody. Data shown are mean ± SEM. n = 14-16. Different letters indicate 75 significant differences at p < 0.05. c) AZD8055 treatment abolishes low [Ca²⁺]-induced pAkt signaling. AZD8055 (1 μM) and rapamycin (10 μM) were used. Representative images are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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