|ZFIN ID: ZDB-PUB-170519-15|
Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action
Liu, C., Dai, W., Bai, Y., Chi, C., Xin, Y., He, G., Mai, K., Duan, C.
|Source:||Scientific Reports 7: 1994 (Journal)|
|Registered Authors:||Duan, Cunming|
|Keywords:||Cell proliferation, Insulin signalling|
|PubMed:||28515443 Full text @ Sci. Rep.|
Liu, C., Dai, W., Bai, Y., Chi, C., Xin, Y., He, G., Mai, K., Duan, C. (2017) Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action. Scientific Reports. 7:1994.
ABSTRACTAberrant regulation of the insulin-like growth factor (IGF)/insulin (IIS)-PI3K-AKT-TOR signaling pathway is linked to major human diseases, and key components of this pathway are targets for therapeutic intervention. Current assays are molecular target- or cell culture-based platforms. Due to the great in vivo complexities inherited in this pathway, there is an unmet need for whole organism based assays. Here we report the development of a zebrafish transgenic line, Tg(igfbp5a:GFP), which faithfully reports the mitotic action of IGF1R-PI3K-Akt-Tor signaling in epithelial cells in real-time. This platform is well suited for high-throughput assays and real-time cell cycle analysis. Using this platform, the dynamics of epithelial cell proliferation in response to low [Ca2+] stress and the distinct roles of Torc1 and Torc2 were elucidated. The availability of Tg(igfbp5a:GFP) line provides a whole organism platform for phenotype-based discovery of novel players and inhibitors in the IIS-PI3K-Akt-Tor signaling pathway.