ZFIN ID: ZDB-FIG-170602-41
Drerup et al., 2017 - Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 10

pJNK and Lamp1 accumulate in p150a/b pLL axon terminals.

(A,B) pJNK accumulates in axon terminals at 4 dpf in p150a/b double mutants. NM3 shown. (C,D) Lamp1 accumulates in NM4 axon terminals at 5 dpf in p150a/b double mutants. Arrows point to axon terminals in wildtype and p150a/b double mutants in all. Axons are labeled by the neurod:egfp BAC transgene (green). Lamp1 and pJNK are show in red in A-D and white in A'-D'. Scale bars = 10 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg standard conditions Day 4 posterior lateral line nerve axon IHC
Day 4 posterior lateral line nerve axon terminus IHC
Day 4 posterior lateral line neuromast axon terminus IHC
nl1Tg ; dctn1as309/s309 ; dctn1bnl17/nl17 standard conditions Day 4 posterior lateral line nerve axon IFL
Day 4 posterior lateral line nerve axon terminus IFL
Day 4 posterior lateral line neuromast axon terminus IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-lamp1 IHC nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
IHC nl1Tg ; dctn1as309/s309 ; dctn1bnl17/nl17 standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
Ab15-mapk IHC nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
IHC nl1Tg ; dctn1as309/s309 ; dctn1bnl17/nl17 standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg ; dctn1as309/s309 ; dctn1bnl17/nl17 standard conditions Day 4 posterior lateral line nerve axon terminus ab15-mapk labeling increased amount, abnormal
Day 4 posterior lateral line nerve axon terminus Ab1-lamp1 labeling increased amount, abnormal
Day 4 posterior lateral line nerve retrograde axonal transport decreased occurrence, abnormal
Day 4 posterior lateral line neuromast axon terminus Ab1-lamp1 labeling increased amount, abnormal
Day 4 posterior lateral line neuromast axon terminus ab15-mapk labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife