ZFIN ID: ZDB-FIG-170602-33
Drerup et al., 2017 - Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 2

Loss of Actr10 causes mitochondrial accumulation in swollen axon terminals.

(A,B) At 4 dpf, swollen axon terminals in actr10 mutants (B) have high levels of Cytochrome c immunolabeling (arrow; NM3 shown) relative to a wildtype sibling (A). (C,D) TagRFP-labeled mitochondria accumulate in actr10 mutant (arrow), but not wildtype, axon terminals at 4 dpf. (E) Mean fluorescence intensity (background subtracted) of Cytochrome C in axon terminals of A and B. (F,G) Mean fluorescence intensity (background subtracted) in axon terminals of H-K show comparable levels of Lamp1 and pJNK fluorescence intensity between wildtype and actr10 mutant terminals (ANOVA; mean ± SEM shown). (H–K) Lamp1 and pJNK immunolabeling in NM3 axon terminals (arrows). Lamp1 and pJNK are in red in (H–K) and white in H'-K'. (L–O) Mitochondrial accumulation in mutant axon terminals, assayed using Cytochrome c immunolabeling (white), can be suppressed by mRNA-mediated expression of mRFP-Actr10. Arrows point to axon terminal regions for comparison. Scale bars = 10 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-cyc IHC actr10nl15/nl15 ; nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
IHC nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
Ab1-lamp1 IHC actr10nl15/nl15 ; nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
IHC nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
Ab15-mapk IHC actr10nl15/nl15 ; nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
IHC nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus
IHC Day 4 posterior lateral line neuromast axon terminus
Phenotype Details
Fish Conditions Stage Phenotype
actr10nl15/nl15 ; nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus Ab1-cyc labeling increased amount, abnormal
Day 4 posterior lateral line nerve axon terminus swollen, abnormal
Day 4 posterior lateral line neuromast axon terminus Ab1-cyc labeling increased amount, abnormal
Day 4 posterior lateral line neuromast axon terminus swollen, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife