FIGURE

Fig. S11

ID

ZDB-FIG-170519-28

Publication

Lei et al., 2017 - Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors

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Fig. S11

Intron-retaining transcripts are located in both nucleus and cytoplasm. RNAscope in situ hybridization assays showing subcellular localization of intron-retaining transcripts on cryosections of fn10a embryos at 36 hpf. DAPI staining (blue) was used to mark the nucleus. (A-A'', B-B'') odc1, as a positive control of RNAscope, was broadly expressed in both siblings (A-A'') and mutants (B-B''). (C-C'', D-D'') dapB, as a negative control of RNAscope, had no signal in siblings (C') and mutants (D'). (E-E'' to H-H'') The intron-containing probes of ccnb1 and tardbp had higher expression in fn10a mutants (F', H') than in siblings (E', G'). Note that retained introns were found in both nucleus and cytoplasm in fn10a mutants although more were found in the nucleus. Arrows indicate their cytoplasmic locations (F'', H''). Scale bars, 10 μm; n ≥5 for each panel.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.