ZFIN ID: ZDB-FIG-170510-3
Kasper et al., 2017 - MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos. Developmental Cell   40:552-565.e5 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4

Enhanced Phenotypic Variability in Hypobranchial Artery Sprouting with Diminishing miR-24 Activity

(A) sox9a WISH labeling chondrocyte progenitors (left) and Alcian blue-stained cartilage (right) in the ventral head of wild-type and miR-24 mutant embryos (n ≥ 6 for all genotypes except n = 2 for quadruple mutants). Numbers indicate the position of pharyngeal arches 1–7. miR-24 genotypes are categorized as in Figure S2B.

(B) Ventral view (25×) of 54-hpf head vasculature. Magnified images depict the spectrum of HA phenotypes (dotted outlines) for each genotype. The HA sprout most representative of the genotype is indicated with a yellow arrow and is depicted in the zoomed-in picture with a yellow HA label. White arrows point to aortic arches (AA).

(C) Bar plots show the average of replicate means ± SEM of HA length (μm) at 54 hpf (n = 4 replicates).

(D) Violin plots show 54-hpf HA length (μm) probability density distributions. Solid lines in box plots depict median values. Phenotypic variability was statistically different from wild-type as determined by the Levene's test (n = 40 embryos). Single: p = 0.04; double: ∗∗∗∗p = 5.77 × 10−6; triple: ∗∗∗p = 3.46 × 10−4; quadruple: ∗∗∗p = 7.57 × 10−4

(E) Bar plots show average replicate SD ± SEM of HA length (μm) at 54 hpf (n = 4 replicates).

For all bar blots, S, single; D, double; T, triple; Q, quadruple mutant. Significance calculations were relative to wild-type embryos. n.s., not significant (p > 0.05); p ≤ 0.05, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, two-tailed Mann-Whitney U test unless otherwise indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP la116Tg control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
la116Tg ; mir24-1ya324/ya324 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
la116Tg ; mir24-1ya324/ya324 ; mir24-2ya325/ya325 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
la116Tg ; mir24-1ya324/ya324 ; mir24-3ya326/ya326 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
la116Tg ; mir24-2ya325/ya325 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
la116Tg ; mir24-4ya327/ya327 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
la116Tg ; mir24-4ya327/ya327 ; mir24-2ya325/ya325 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
la116Tg ; mir24-4ya327/ya327 ; mir24-3ya326/ya326 ; mir24-2ya325/ya325 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
mir24-1ya324/ya324 ; la116Tg ; mir24-2ya325/ya325 ; mir24-4ya327/ya327 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
mir24-1ya324/ya324 ; la116Tg ; mir24-4ya327/ya327 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
mir24-2ya325/ya325 ; la116Tg ; mir24-3ya326/ya326 ; mir24-1ya324/ya324 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
mir24-2ya325/ya325 ; mir24-1ya324/ya324 ; mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
mir24-3ya326/ya326 ; la116Tg control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 ; mir24-1ya324/ya324 control Long-pec aortic arch IHC
Long-pec cranial vasculature IHC
sox9a AB control Long-pec head chondroblast ISH
la116Tg ; mir24-1ya324/ya324 control Long-pec head chondroblast ISH
la116Tg ; mir24-1ya324/ya324 ; mir24-2ya325/ya325 control Long-pec head chondroblast ISH
la116Tg ; mir24-1ya324/ya324 ; mir24-3ya326/ya326 control Long-pec head chondroblast ISH
la116Tg ; mir24-2ya325/ya325 control Long-pec head chondroblast ISH
la116Tg ; mir24-4ya327/ya327 control Long-pec head chondroblast ISH
la116Tg ; mir24-4ya327/ya327 ; mir24-2ya325/ya325 control Long-pec head chondroblast ISH
la116Tg ; mir24-4ya327/ya327 ; mir24-3ya326/ya326 ; mir24-2ya325/ya325 control Long-pec head chondroblast ISH
mir24-1ya324/ya324 ; la116Tg ; mir24-2ya325/ya325 ; mir24-4ya327/ya327 control Long-pec head chondroblast ISH
mir24-1ya324/ya324 ; la116Tg ; mir24-4ya327/ya327 control Long-pec head chondroblast ISH
mir24-2ya325/ya325 ; la116Tg ; mir24-3ya326/ya326 ; mir24-1ya324/ya324 control Long-pec head chondroblast ISH
mir24-2ya325/ya325 ; mir24-1ya324/ya324 ; mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 control Long-pec head chondroblast ISH
mir24-3ya326/ya326 ; la116Tg control Long-pec head chondroblast ISH
mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 control Long-pec head chondroblast ISH
mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 ; mir24-1ya324/ya324 control Long-pec head chondroblast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la116Tg ; mir24-1ya324/ya324 control Long-pec hypobranchial artery increased variability of size, abnormal
la116Tg ; mir24-1ya324/ya324 ; mir24-2ya325/ya325 control Long-pec hypobranchial artery increased variability of size, abnormal
la116Tg ; mir24-1ya324/ya324 ; mir24-3ya326/ya326 control Long-pec hypobranchial artery increased variability of size, abnormal
la116Tg ; mir24-2ya325/ya325 control Long-pec hypobranchial artery increased variability of size, abnormal
la116Tg ; mir24-4ya327/ya327 control Long-pec hypobranchial artery increased variability of size, abnormal
la116Tg ; mir24-4ya327/ya327 ; mir24-2ya325/ya325 control Long-pec hypobranchial artery increased variability of size, abnormal
la116Tg ; mir24-4ya327/ya327 ; mir24-3ya326/ya326 ; mir24-2ya325/ya325 control Long-pec hypobranchial artery decreased length, abnormal
Long-pec hypobranchial artery increased variability of size, abnormal
mir24-1ya324/ya324 ; la116Tg ; mir24-2ya325/ya325 ; mir24-4ya327/ya327 control Long-pec hypobranchial artery decreased length, abnormal
Long-pec hypobranchial artery increased variability of size, abnormal
mir24-1ya324/ya324 ; la116Tg ; mir24-4ya327/ya327 control Long-pec hypobranchial artery increased variability of size, abnormal
mir24-2ya325/ya325 ; la116Tg ; mir24-3ya326/ya326 ; mir24-1ya324/ya324 control Long-pec hypobranchial artery decreased length, abnormal
Long-pec hypobranchial artery increased variability of size, abnormal
mir24-2ya325/ya325 ; mir24-1ya324/ya324 ; mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 control Long-pec hypobranchial artery decreased length, abnormal
Long-pec hypobranchial artery increased variability of size, abnormal
mir24-3ya326/ya326 ; la116Tg control Long-pec hypobranchial artery increased variability of size, abnormal
mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 control Long-pec hypobranchial artery increased variability of size, abnormal
mir24-4ya327/ya327 ; la116Tg ; mir24-3ya326/ya326 ; mir24-1ya324/ya324 control Long-pec hypobranchial artery decreased length, abnormal
Long-pec hypobranchial artery increased variability of size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 40, Kasper, D.M., Moro, A., Ristori, E., Narayanan, A., Hill-Teran, G., Fleming, E., Moreno-Mateos, M., Vejnar, C.E., Zhang, J., Lee, D., Gu, M., Gerstein, M., Giraldez, A., Nicoli, S., MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos, 552-565.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell