ZFIN ID: ZDB-FIG-170317-2
Schultz et al., 2017 - Vascular Endothelial Growth Factor A and Leptin Expression Associated with Ectopic Proliferation and Retinal Dysplasia in Zebrafish Optic Pathway Tumors. Zebrafish   14(4):343-356 Full text @ Zebrafish
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 2

Ectopic proliferation and Sox2 expressing cells in young adult Tg(flk1:RFP)is18 dysplastic retina. (a–e) Wild-type 4-month-old adult (n = 3) shows that PCNA positive cells are restricted to stem/progenitor cells at the ciliary marginal zone (cmz, arrow, c) and rod precursor cells in the photoreceptor outer nuclear layer (arrowheads, e). Sox2-expressing cells overlap with PCNA positive stem cells at the ciliary marginal zone (bracket, c) and are present in amacrine/displaced amacrine cells in the inner nuclear and ganglion cell layers (c, e). (f–j) Retina from 4-month-old Tg(flk1:RFP)is18 adult (n = 3) shows numerous regions of proliferation distributed throughout the neural retina (f). The ciliary marginal zone (g, h) and ventral ciliary circumferential artery (asterisk, g) are expanded. Small masses of PCNA and Sox2 positive cells (arrowheads, g, h) are present in the normally single-cell layered nonpigmented epithelium extending from the ciliary marginal zone (small arrow, g, h–compare to small arrow in wild type b). Ectopic regions of proliferation in the neural retina contain disorganized cells expressing PCNA and Sox2 (arrowheads, j). cmz, ciliary marginal zone; gcl, ganglion cell layer; inl, inner nuclear layer; onl, outer nuclear layer; pe, pigmented epithelium. Scale bars (a, f) 200 μm; all other panels 50 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-sox2 IHC WIK standard conditions Adult amacrine cell
IHC Adult Muller cell
IHC si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult ciliary marginal zone
IHC Adult retina
Ab2-pcna IHC WIK standard conditions Adult ciliary marginal zone
IHC si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult ciliary marginal zone
IHC Adult retina
Phenotype Details
Fish Conditions Stage Phenotype
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult ciliary marginal zone ab2-pcna labeling increased amount, abnormal
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult ciliary marginal zone ab1-sox2 labeling increased amount, abnormal
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult ciliary marginal zone increased size, abnormal
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina ab1-sox2 labeling increased amount, abnormal
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina ab2-pcna labeling increased amount, abnormal
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retinal neural layer has extra parts of type proliferative region, abnormal
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult ventral branch nasal ciliary artery increased diameter, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Zebrafish for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Zebrafish