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ZFIN ID: ZDB-FIG-170317-3
Schultz et al., 2017 - Vascular Endothelial Growth Factor A and Leptin Expression Associated with Ectopic Proliferation and Retinal Dysplasia in Zebrafish Optic Pathway Tumors. Zebrafish   14(4):343-356 Full text @ Zebrafish
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

Induction of vegfab and lepb expression in distinct cell populations in Tg(flk1:RFP)is18 dysplastic retina and retinal tumor. In situ hybridization of retina cryosections from +/+ (a, b, e, f, i, j, m, n, q, r, u, v) and Tg(flk1:RFP)is18 (c, d, g, h, k, l, o, p, s, t, w, x) adult siblings. Weak expression of vegfaa is detected in the three nuclear layers of the retina (a, b) and in early dysplastic (c [box], d) and retinal tumor tissue (c, asterisk). Expression of vegfab (e, f), the VEGF receptor flk1 (i, j), lepb (m, n), or the Leptin receptor lepr (q, r) is not detected in wild-type +/+ retina. (g, h) In early dysplastic retina vegfab is detected throughout the inner and outer nuclear layers (g [small bracket], h). In the tumor containing region (large bracket) vegfab is detected in a central region of the lesion (asterisk). (k, l) The VEGF receptor flk1 was present in small groups of cells scattered throughout the tissue that may overlap with microvessels. (o, p) lepb is highly expressed in a subset of cells in the inner nuclear layer and cells in the ganglion cell layer in dysplastic retina and present in many cells evenly distributed in the early tumor (bracket). (s, t) The lepr receptor expression is faint and diffuse in dysplastic retina and tumor tissue. (u, v) In wild-type retina the glial marker apoeb is strongly expressed in inner nuclear layer Müller glia and astrocytes in the ganglion cell layer/nerve fiber layer. (w, x) apoeb expression is highly elevated in dysplastic retina and is detected in projections crossing the inner plexiform layer. Numerous cells throughout the tumor tissue labeled intensely with apoeb. gcl, ganglion cell layer; inl, inner nuclear layer; onl, outer nuclear layer; pe, pigmented epithelium. All scale bars, 100 μm, except panels (g, k, o, s, t) scale bars, 500 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apoeb WIK standard conditions Adult retinal ganglion cell layer ISH
Adult retinal inner nuclear layer ISH
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina neoplasm ISH
Adult retinal ganglion cell layer ISH
Adult retinal inner nuclear layer ISH
kdrl WIK standard conditions Adult Not Detected retinal neural layer ISH
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina neoplasm ISH
lepb WIK standard conditions Adult Not Detected retinal neural layer ISH
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult astrocyte ISH
Adult retina neoplasm ISH
Adult retinal inner nuclear layer Muller cell ISH
lepr WIK standard conditions Adult Not Detected retinal neural layer ISH
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina neoplasm ISH
vegfaa WIK standard conditions Adult retinal neural layer ISH
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina ISH
Adult retina neoplasm ISH
vegfab WIK standard conditions Adult Not Detected retinal neural layer ISH
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina neoplasm ISH
Adult Not Detected retinal ganglion cell layer ISH
Adult retinal inner nuclear layer ISH
Adult retinal outer nuclear layer ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
si:ch211-246i5.4is18Tg/+(WIK) standard conditions Adult retina vegfaa expression increased amount, abnormal
Adult retinal ganglion cell layer apoeb expression increased amount, abnormal
Adult retinal inner nuclear layer apoeb expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Zebrafish for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Zebrafish