ZFIN ID: ZDB-FIG-170227-30
Jao et al., 2017 - A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies. Molecular biology of the cell   28:120-127 Full text @ Mol. Biol. Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Pec-fin

Fig. 4

Depletion of Gle1 reduces ciliary beating and alters left–right asymmetry in zebrafish. (A) The axoneme and the basal body of the cilia were mosaically labeled by Arl13b-eGFP and Cetn4-TagRFP, respectively, in uninjected, ipk1MO1-injected, or gle1MOs-injected embryos. Motile cilia in the Kupffer’s vesicle were imaged at six- to nine-somite stages from the dorsal side, anterior to the right. Normal ciliary beating is detected by a fan-like morphology in the control. Scale bar, 5 μm. (B) Tg(-5.1mnx1:TagRFP) embryos were injected with suboptimal doses of translation-blocking ipk1 morpholino (ipk1MO1, 4 ng), a mix of two nonoverlapping translation-blocking gle1 morpholinos (gle1MOs, 1.5 ng each), or a combination of both ipk1MO1 and gle1MOs at the one-cell stage. The direction of the heart looping (TagRFP+) was assessed at 60–72 h post fertilization. Three independent injections were performed for each condition. Values are mean ± SEM; n is the number of embryos analyzed in each condition.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-gle1 + MO2-gle1 control 5-9 somites Kupffer's vesicle cilium movement decreased rate, abnormal
AB + MO1-ippk control 5-9 somites Kupffer's vesicle cilium movement decreased rate, abnormal
vu504Tg + MO1-gle1 + MO1-ippk + MO2-gle1 standard conditions Pec-fin determination of heart left/right asymmetry disrupted, abnormal
Pec-fin heart looping disrupted, abnormal
Pec-fin heart tube position, abnormal
vu504Tg + MO1-gle1 + MO2-gle1 control Pec-fin determination of heart left/right asymmetry disrupted, abnormal
Pec-fin heart looping disrupted, abnormal
Pec-fin heart tube position, abnormal
vu504Tg + MO1-ippk control Pec-fin determination of heart left/right asymmetry disrupted, abnormal
Pec-fin heart looping disrupted, abnormal
Pec-fin heart tube position, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular biology of the cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Biol. Cell