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ZFIN ID: ZDB-FIG-170110-3
Fehr et al., 2016 - A Zebrafish Model for Chlamydia Infection with the Obligate Intracellular Pathogen Waddlia chondrophila.. Frontiers in microbiology   7:1829 Full text @ Front Microbiol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 4

Light-microscope appearance of a transgenic Tg(fli1a:eGFP) larva at 48 hpi after intravenous injection of live bacteria (A), showing an impaired blood flow ending in an almost completely stopped circulation, accompanied by pericardial oedema formation (arrow). Section view of a CLSM acquired 3D stack (B) after IF staining with an anti-Waddlia antibody, showing the infection of GFP expressing endothelial cells (green) with W. chondrophila (red). Host cell nuclei were stained with DAPI (blue). Surface rendering of the tail artery and caudal vein shows the distribution of the inclusions inside the vasculature at 24 hpi (C) and 36 hpi (D), showing bacterial spread across endothelial bounds (arrows).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg bacterial treatment by injection: Waddlia chondrophila WSU 86-1044 Protruding-mouth vasculature IHC
Day 4 vasculature IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg bacterial treatment by injection: Waddlia chondrophila WSU 86-1044 Day 4 blood circulation decreased process quality, abnormal
Day 4 pericardium edematous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in microbiology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Microbiol