ZFIN ID: ZDB-FIG-161208-2
Schulz et al., 2016 - Critical role for adenosine receptor A2a in β-cell proliferation. Molecular metabolism   5:1138-1146 Full text @ Mol Metab
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 1

Pharmacological activation of adenosine signaling promotes β-cell proliferation independently of an apoptotic microenvironment. (A) Schema of the analysis of β-cell proliferation in control zebrafish larvae and larvae subjected to β-cell ablation or δ-cell ablation. Ablation was carried out by exposing the nitroreductase (NTR)-expressing transgenics to metronidazole (MTZ) from 3 to 4 days post fertilization (dpf). Islets were analyzed by confocal microscopy at 5 dpf. (B–G) Representative pictures of proliferating β-cells (arrowheads) in islets of Tg(ins:venus-geminin) zebrafish treated with DMSO or NECA from 4 to 5 dpf in the presence or absence of β-cell ablation (D–E) or δ-cell ablation (F–G). β-cell ablation was achieved by crossing the Tg(ins:venus-geminin) zebrafish with Tg(ins:flag-NTR) zebrafish and treating them with MTZ; δ-cell ablation by crossing them with Tg(sst:NTR) zebrafish and treating them with MTZ. (H) β-cell proliferation in the absence of cell ablation (CTL), and after β-cell or δ-cell ablation. Each condition was normalized (DMSO = 1), allowing comparison of fold changes. Absolute numbers are shown in Figure S1. n = 13–32; scale bars: 10 μm; **p < 0.01; ****p < 0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Venus ki102Tg ; ki107Tg chemical ablation: pancreatic D cell, chemical treatment by environment: metronidazole Day 5 pancreatic B cell IFL
ki102Tg ; ki107Tg chemical ablation: pancreatic D cell, chemical treatment by environment: metronidazole, chemical treatment: adenosine receptor agonist Day 5 pancreatic B cell IFL
ki107Tg chemical treatment by environment: metronidazole Day 5 pancreatic B cell IFL
ki107Tg chemical treatment by environment: metronidazole, chemical treatment: adenosine receptor agonist Day 5 pancreatic B cell IFL
ki107Tg ; s950Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Day 5 pancreatic B cell IFL
ki107Tg ; s950Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole, chemical treatment: adenosine receptor agonist Day 5 pancreatic B cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ki107Tg chemical treatment by environment: metronidazole, chemical treatment: adenosine receptor agonist Day 5 pancreatic B cell cell population proliferation increased occurrence, abnormal
ki107Tg ; s950Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole, chemical treatment: adenosine receptor agonist Day 5 pancreatic B cell cell population proliferation increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular metabolism for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol Metab