header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-160726-37
Barber et al., 2016 - Similarly Lethal Strains of Extraintestinal Pathogenic Escherichia coli Trigger Markedly Diverse Host Responses in a Zebrafish Model of Sepsis. mSphere   1(2) Full text @ mSphere
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Pec-fin
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Pec-fin

Fig. 2

Distinct pathologies associated with different but equally lethal ExPEC isolates. (A) An F11-infected embryo displaying characteristic pericardial edema (arrowhead) at 12 hpi. This is not seen following the injection of controls with PBS (left). (B) A CFT073-infected embryo at 12 hpi showing fin erosion and ulceration commonly seen during infection with either CFT073 or F11. (C) F11-infected embryos, but not those infected with CFT073, often develop protrusions (arrowhead) in the trunk or tail region by 12 hpi. (D) Merged bright-field and fluorescent images of an F11/pGEN-GFP(LVA)-infected embryo at 12 hpi showing bacteria at the base of a protrusion (inset) but not within the main structure. (E) Bright-field and matched fluorescent images of F11-infected embryos stained with acridine orange (green) at 12 hpi. The dye accumulates within protrusions on the trunk (left, Chevron) and tail (right, arrows). (F) Bright-field (top) or fluorescent (bottom) images of Tg(krt8:GFP) embryos at 12 hpi with F11. Epithelial cells within this transgenic line express GFP. Each arrowhead marks an F11-induced protrusion. (G) Merged bright-field and fluorescent images of Tg(fli1a:GFP) embryos at 12 hpi with F11. Endothelial cells and leukocytes in this transgenic line express GFP. Protrusions are indicated by arrowheads.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP gz7Tg bacterial treatment by injection: Escherichia coli Pec-fin epithelial cell IFL
y1Tg bacterial treatment by injection: Escherichia coli Pec-fin endothelial cell IFL
Pec-fin leukocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT bacterial treatment by injection: Escherichia coli Pec-fin pericardium edematous, abnormal
gz7Tg bacterial treatment by injection: Escherichia coli Pec-fin epithelial cell protruding, abnormal
y1Tg bacterial treatment by injection: Escherichia coli Pec-fin epithelial cell protruding, abnormal
Acknowledgments:
ZFIN wishes to thank the journal mSphere for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ mSphere