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ZIRC
ZFIN ID: ZDB-FIG-160525-41
Sulaiman et al., 2016 - A novel small molecule ameliorates ocular neovascularisation and synergises with anti-VEGF therapy. Scientific Reports   6:25509 Full text @ Sci. Rep.
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 2

SH-11037 significantly inhibits ocular developmental angiogenesis in zebrafish larvae.

(a) Representative epi-fluorescence images of hyaloid vessel (HV) morphology on lenses dissected from drug-treated 5 days post fertilisation (dpf) Tg(fli1:EGFP) larvae. A significant reduction in primary HV branch number is observed at 10 µM SH-11037 (white arrows), scale bars = 50 µm. (b) Representative bright-field images of 5 dpf larval morphology following SH-11037 treatment. Some larvae treated with 10 µM SH-11037 demonstrated pericardial and yolk sac oedema. Scale bars = 500 µm. (c) Quantification of the dose-dependent inhibition of primary HV development in 5 dpf Tg(fli1:EGFP) larvae treated from 2 dpf with 1-10 µM SH-11037. (d) Quantification of the dose-dependent inhibition of SH-11037 on number of hyaloid vessel branches in treated larvae compared to DMSO control. Up to a 40% reduction in primary HV and vessel branches is observed in response to 10 µM SH-11037. ***P <0.01, ***P < 0.001, one-way ANOVA with Dunnett’s post hoc tests. Mean ± s.e.m., n ≥ 3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Day 5 hyaloid vessel IFL
y1Tg chemical treatment: SH-11037 Day 5 hyaloid vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: SH-11037 Day 5 hyaloid vessel decreased branchiness, abnormal
Day 5 hyaloid vessel angiogenesis decreased occurrence, abnormal
Day 5 pericardium edematous, abnormal
Day 5 yolk edematous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.