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ZFIN ID: ZDB-FIG-160525-41
Sulaiman et al., 2016 - A novel small molecule ameliorates ocular neovascularisation and synergises with anti-VEGF therapy. Scientific Reports   6:25509 Full text @ Sci. Rep.
Anatomical Term:
Stage: Day 5
Observed In:
Stage: Day 5

Fig. 2

SH-11037 significantly inhibits ocular developmental angiogenesis in zebrafish larvae.

(a) Representative epi-fluorescence images of hyaloid vessel (HV) morphology on lenses dissected from drug-treated 5 days post fertilisation (dpf) Tg(fli1:EGFP) larvae. A significant reduction in primary HV branch number is observed at 10 µM SH-11037 (white arrows), scale bars = 50 µm. (b) Representative bright-field images of 5 dpf larval morphology following SH-11037 treatment. Some larvae treated with 10 µM SH-11037 demonstrated pericardial and yolk sac oedema. Scale bars = 500 µm. (c) Quantification of the dose-dependent inhibition of primary HV development in 5 dpf Tg(fli1:EGFP) larvae treated from 2 dpf with 1-10 µM SH-11037. (d) Quantification of the dose-dependent inhibition of SH-11037 on number of hyaloid vessel branches in treated larvae compared to DMSO control. Up to a 40% reduction in primary HV and vessel branches is observed in response to 10 µM SH-11037. ***P <0.01, ***P < 0.001, one-way ANOVA with Dunnett’s post hoc tests. Mean ± s.e.m., n ≥ 3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Day 5 hyaloid vessel IFL
y1Tg chemical treatment: SH-11037 Day 5 hyaloid vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: SH-11037 Day 5 hyaloid vessel decreased branchiness, abnormal
Day 5 hyaloid vessel angiogenesis decreased occurrence, abnormal
Day 5 pericardium edematous, abnormal
Day 5 yolk edematous, abnormal
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.