FIGURE

Fig. 4

ID

ZDB-FIG-160421-1

Publication

Chiavacci et al., 2015 - MicroRNA 19a replacement partially rescues fin and cardiac defects in zebrafish model of Holt Oram syndrome

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

miR-19a dysregulation affects zebrafish heart development.

(A,B) Tg(Myl7:EGFP) embryos were injected with increasing amounts of miR-Ct or miR-19a mimics. (A) Images of representative 72 hpf Tg(Myl7:EGFP) embryo phenotypes. (B) Percentage of embryos with the indicated phenotypes, averaged across multiple independent experiments carried out in double blind. (D,E) Phenotypes of 72 hpf Tg(Myl7:EGFP) embryos co-injected with 0.5 ng of MO-Tbx5a and with 8ng of MO-19a or MO-Ct. N, total numbers of analyzed embryos. Scale bar 100 μm. hts: heartstring phenotype; mild defects: general cardiac defects; WT: wild type heart. NO fins: pectoral fins absence, WT fins: wild type pectoral fins. P values by Fisher’s test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	twu34Tg + MO2-dre-mir-19a twu34Tg + MO1-tbx5a + MO2-dre-mir-19a
Knockdown Reagents:	MO1-tbx5a MO2-dre-mir-19a
Observed In:	embryo development fin heart heart development
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.