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ZFIN ID: ZDB-FIG-160411-13
Marra et al., 2016 - Epithelial cell fate in the nephron tubule is mediated by the ETS transcription factors etv5a and etv4 during zebrafish kidney development. Developmental Biology   411(2):231-45 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4

Redundancy between etv5a and etv4 in MCC formation. (A) In embryos injected with either etv5a MO, etv4 MO, or a combination of etv5a MO and etv4 MO, there is reduced expression of odf3b (purple), where the greatest loss appears in the absence of both etv5a and etv4 transcripts. (B) A significant decrease in average MCC number results in the loss of etv5a, as well as the loss of etv4. Injection of etv5a and etv4 together caused a greater decrease in the average number of MCCs. Representative images are shown for the quantified groups (n>50 for each), and standard error is depicted by the error bars. p-values: *****p<0.001, ****p<0.002.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
odf3b WT standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
WT + MO1-etv5a standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
WT + MO1-etv5a + MO2-etv4 standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
WT + MO2-etv4 standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-etv5a standard conditions Prim-5 pronephric nephron tubule epithelial cell differentiation process quality, abnormal
Prim-5 pronephros has fewer parts of type pronephros multi-ciliated epithelial cell, abnormal
Prim-5 pronephros multi-ciliated epithelial cell differentiation decreased occurrence, abnormal
WT + MO1-etv5a + MO2-etv4 standard conditions Prim-5 pronephric nephron tubule epithelial cell differentiation process quality, abnormal
Prim-5 pronephros has fewer parts of type pronephros multi-ciliated epithelial cell, abnormal
Prim-5 pronephros multi-ciliated epithelial cell differentiation decreased occurrence, abnormal
WT + MO2-etv4 standard conditions Prim-5 pronephric nephron tubule epithelial cell differentiation process quality, abnormal
Prim-5 pronephros has fewer parts of type pronephros multi-ciliated epithelial cell, abnormal
Prim-5 pronephros multi-ciliated epithelial cell differentiation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 411(2), Marra, A.N., Wingert, R.A., Epithelial cell fate in the nephron tubule is mediated by the ETS transcription factors etv5a and etv4 during zebrafish kidney development, 231-45, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.