Regulation of microtubule dynamics is required for NC. a Dorsal views of untreated, nocodazole-treated (5 µg/ml) and paclitaxel-treated (50 µM) embryos labeled by in situ hybridization with the dlx3 riboprobe. Double red arrowheads indicate the width of the neural plate. Scale bar: 100 µm . b Quantification of the neural plate width (µm) in control (untreated) and drug-treated embryos. (*) indicates statistical significance (P <0.001 for untreated vs nocodazole and untreated vs paclitaxel) using a Kruskal-Wallis test followed by Dunn’s post-hoc test. c Quantification of the length-to-width (LWR) ratio of mGFP-labeled cells in control (untreated), nocodazole-treated, and paclitaxel-treated embryos at the 4–5 som stage. (*) indicates statistical significance (P <0.001 for untreated vs nocodazole and P <0.01 for untreated vs paclitaxel) using a Kruskal-Wallis test followed by Dunn’s post-hoc test. d Hindbrain sections of 4–5 som control (untreated), nocodazole-treated and paclitaxel-treated embryos mosaically expressing mGFP (green). Nuclei are labeled in blue with DAPI. Double arrows indicate cell length. The dotted white line represents the midline. Scale bar: 10 µm
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