ZFIN ID: ZDB-FIG-160229-6
Jungke et al., 2016 - Generation of a conditional lima1a allele in zebrafish using the FLEx switch technology. Genesis (New York, N.Y. : 2000)   54(1):19-28 Full text @ Genesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 3

Functional consequences of the integration of SAGFLEx into the lima1a locus. (A) Schematic representation of SAGFLEx integrated into intron 2 of the lima1a locus in Tg(FLEx-lima1a:GFP). The positions of the primers used for qRT-PCR analysis (GFP-for/GFP-rev, E9-for/E9-rev) and the lima1a exon 9 probe are indicated. (B) Mating of heterozygous Tg(FLEx-lima1a:GFP) animals results in offspring with no, medium and strong GFP fluorescence. (CE) Expression of lima1a in embryos with no, medium and strong GFP fluorescence (no GFP, GFP+, GFP++) using an exon 9 probe. Lateral and dorsal views at 28 hpf. (F) qRT-PCR analysis of lima1a exon 9 expression in embryos with medium and strong GFP fluorescence compared to siblings with no GFP fluorescence. ***P value ≥ 0,001. (G, H) Cellular localization of GFP fusions with wildtype Lima1a encoded by exons 1–9 (E1–E9) and truncated Lima1a encoded by exons 1–2 (E1–E2).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP lima1atud39Gt/+(AB) standard conditions Prim-5 forebrain IHC
Prim-5 hindbrain IHC
Prim-5 midbrain IHC
lima1atud39Gt/tud39Gt(AB) standard conditions Prim-5 forebrain IHC
Prim-5 hindbrain IHC
Prim-5 midbrain IHC
lima1a WT standard conditions Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
lima1atud39Gt/+(AB) standard conditions Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
lima1atud39Gt/tud39Gt(AB) standard conditions Prim-5 Not Detected forebrain ISH
Prim-5 Not Detected hindbrain ISH
Prim-5 Not Detected midbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lima1atud39Gt/+(AB) standard conditions Prim-5 forebrain lima1a expression decreased amount, abnormal
Prim-5 hindbrain lima1a expression decreased amount, abnormal
Prim-5 midbrain lima1a expression decreased amount, abnormal
lima1atud39Gt/tud39Gt(AB) standard conditions Prim-5 forebrain lima1a expression absent, abnormal
Prim-5 hindbrain lima1a expression absent, abnormal
Prim-5 midbrain lima1a expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genesis (New York, N.Y. : 2000) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genesis