Fig. 7

Depletion of z-miR-9 and z-miR-200-class in zebrafish embryos affects differentiation and migration of GnRH + neurons. a. Scheme showing the positions of the GnRH3::GFP + neurons (green), relative to the OPL, the OB and the olfactory nerves (yellow and blue), in a frontal view. The anterior commissure is shown at the basis of the OB. On the top is the scheme illustrating the view plane (frontal). AC, Anterior Commissure; GL, Glomeruli; OB, Olfactory Bulb; OPL, Olfactory Placode. b. Scheme illustrating the view plane (ventral) used for the fluorescent images. c–h. Micrographs of GnRH3:GFP zebrafish embryos not injected (c), injected with anti-z-miR-9 MO (d, e), or with anti-z-miR-200 class (f–h). Insets show low-magnifications merged micrographs, for reference. In not injected or control MO-injected embryos, no significant alteration was observed. White arrows indicate the normal position of the GFP + neurons. Red arrows and asterisks indicate, respectively, mispositioned GFP + neurons and loss of neurons. j. Quantification of the observed phenotypes (top, reduced number of neurons; bottom, scattered position) expressed as percent of the injected GFP + embryos showing the indicated phenotype, over the total number of GFP + embryos examined, expressed as %. k. Whole-mount bright field micrographs of control and injected embryo, showing normal embryonic morphology and growth.