Fig. 6

Depletion of z-miR-200-class in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), or injected with control MO, or injected with anti-z-miR-200 class (right panels) MO. The control MO did not cause significant alterations. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing normal embryo morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Proportions of embryos showing either placode disorganization, olfactory axon mistargeting, or both (last bars) after injection of control or z-miR-200 class MOs. e. Quantification of endogenous miR-200 class in embryos injected with control or anti-z-miR-200 MOs, by Real-Time qPCR. Results show a significant decrease of miR-200 abundance in the injected embryos. f. Quantification of endogenous z-foxg1 mRNA in zebrafish embryos injected with anti-z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-200 causes a significant increase of z-foxg1 mRNA abundance. g. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti-miR-200 class MOs, relative to control MO injected embryos, set = 1. The abundance of z-hoxA7a and abundance of z-hoxA10b were also determined and used as in Fig. 5.